PMID- 10965121
OWN - NLM
STAT- MEDLINE
DCOM- 20000921
LR  - 20171101
IS  - 0301-0171 (Print)
IS  - 0301-0171 (Linking)
VI  - 89
IP  - 3-4
DP  - 2000
TI  - Direct visualization of the genomic distribution and organization of two cervid 
      centromeric satellite DNA families.
PG  - 192-8
AB  - Several repetitive DNA fragments were generated from PCR amplifications of 
      caribou DNA using primer sequences derived from the white-tailed deer satellite 
      II DNA clone OvDII. Two fragments, designated Rt-0.5 and Rt-0.7, were sequenced 
      and found to have 96% sequence similarity. These caribou clones also had 85% 
      sequence similarity with OvDII. Multiple-colored fluorescence in situ 
      hybridization (FISH) studies with satellite I and satellite II DNA probes to 
      caribou metaphase chromosomes and extended chromatin fibers provided direct 
      visualization of the genomic organization of these two satellite DNA families, 
      with the following findings: (1) Cervid satellite I DNA is confined to the 
      centromeric regions of the acrocentric autosomes, whereas satellite II DNA is 
      found at the centromeric regions of all chromosomes except for the Y. (2) For 
      most acrocentric chromosomes, the satellite I signal appeared to be medially 
      located at the primary constriction, in contrast to that of satellite II, which 
      appeared to be oriented toward the lateral sides as two separate fluorescent 
      dots. (3) The satellite II clone Rt-0.7 appeared to be enriched in the 
      centromeric region of the caribou X chromosome, a pair of biarmed autosomes, and 
      a number of other acrocentric autosomes. (4) Fiber-FISH demonstrated that the 
      satellite I and satellite II arrays were juxtaposed. On highly extended chromatin 
      fibers, the total length of the hybridization signals for the two satellite DNA 
      arrays often reached 300-400 microm. The length of a given satellite II array 
      usually reached 200 microm, corresponding to 2 x 10(3) kb of DNA in a given 
      centromere.
CI  - Copyright 2000 S. Karger AG, Basel.
FAU - Li, Y C
AU  - Li YC
AD  - Department of Life Sciences, Chung Shan Medical and Dental College, Taichung, 
      Taiwan.
FAU - Lee, C
AU  - Lee C
FAU - Hseu, T H
AU  - Hseu TH
FAU - Li, S Y
AU  - Li SY
FAU - Lin, C C
AU  - Lin CC
LA  - eng
SI  - GENBANK/AF170124
SI  - GENBANK/AF170125
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Switzerland
TA  - Cytogenet Cell Genet
JT  - Cytogenetics and cell genetics
JID - 0367735
RN  - 0 (DNA, Satellite)
SB  - IM
EIN - Cytogenet Cell Genet 2001;92(1-2):73. Hsu TH [corrected to Hseu TH]
MH  - Animals
MH  - Base Sequence
MH  - Blotting, Southern
MH  - Centromere/*genetics
MH  - Chromosome Mapping
MH  - DNA, Satellite/chemistry/*genetics/isolation & purification
MH  - Deer/*genetics
MH  - Gene Dosage
MH  - *Genome
MH  - In Situ Hybridization, Fluorescence
MH  - Molecular Sequence Data
MH  - Sequence Alignment
MH  - Sequence Analysis, DNA
MH  - Sequence Homology, Nucleic Acid
EDAT- 2000/08/31 11:00
MHDA- 2000/09/23 11:01
CRDT- 2000/08/31 11:00
PHST- 2000/08/31 11:00 [pubmed]
PHST- 2000/09/23 11:01 [medline]
PHST- 2000/08/31 11:00 [entrez]
AID - 15611 [pii]
AID - 10.1159/000015611 [doi]
PST - ppublish
SO  - Cytogenet Cell Genet. 2000;89(3-4):192-8. doi: 10.1159/000015611.