PMID- 10965231
OWN - NLM
STAT- MEDLINE
DCOM- 20001006
LR  - 20171101
IS  - 1021-7401 (Print)
IS  - 1021-7401 (Linking)
VI  - 8
IP  - 2
DP  - 2000
TI  - Peripheral effects of methionine-enkephalin on inflammatory reactions and 
      behavior in the rat.
PG  - 70-7
AB  - Methionine-enkephalin (Met-Enk) induces notable alterations in immune and central 
      nervous system functions. The present study was conducted in order to compare 
      peripheral and central effects of Met-Enk on nonspecific immunity, open field 
      behavior and pain perception in the rat. The results showed that 0.2 mg/kg of 
      Met-Enk given intraperitoneally (i.p.) increased concanavalin A (Con-A)-induced 
      paw edema and enhanced basal and phorbol myristate acetate (PMA)-stimulated 
      H(2)O(2) production of peritoneal macrophages. Met-Enk-induced immunopotentiation 
      was antagonized by anti-Met-Enk antibodies (anti-Met-Enk-Ig) and quaternary 
      naltrexone (qNtx). Met-Enk injected i.p. produced an increase of horizontal and 
      vertical locomotor activity in the open field that was reversed by i. p. 
      administration of anti-Met-Enk-Ig and qNtx. The dose of 0.2 mg/kg of Met-Enk 
      applied i.p. did not affect the number of writhes in the test of analgesia. 
      Intracerebroventricular (i.c.v.) injection of Met-Enk, given in a dose that was 
      previously shown to be immunostimulatory, enhanced only basal H(2)O(2) production 
      of peritoneal macrophages, and anti-Met-Enk-Ig antagonized this effect. Besides, 
      i.c.v. treatment with anti-Met-Enk-Ig increased and decreased H(2)O(2) production 
      of peritoneal macrophages under basal and stimulated conditions, respectively. 
      Met-Enk and anti-Met-Enk-Ig injected i.c.v. did not influence activity in the 
      open field and pain sensitivity. Thus, the i.c.v. dose of Met-Enk that was 
      sufficient to modulate immune functions did not influence behavior. It may be 
      concluded that Met-Enk modulated nonspecific immune responses and open field 
      behavior by peripheral mechanisms.
CI  - Copyright 2000 S. Karger AG, Basel
FAU - Vujic-Redzic, V
AU  - Vujic-Redzic V
AD  - Immunology Research Center 'Branislav Jankovic', School of Medicine, Belgrade, 
      Yugoslavia.
FAU - Dimitrijevic, M
AU  - Dimitrijevic M
FAU - Stanojevic, S
AU  - Stanojevic S
FAU - Kovacevic-Jovanovic, V
AU  - Kovacevic-Jovanovic V
FAU - Miletic, T
AU  - Miletic T
FAU - Radulovi J
AU  - Radulovi J
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Switzerland
TA  - Neuroimmunomodulation
JT  - Neuroimmunomodulation
JID - 9422763
RN  - 0 (Antibodies)
RN  - 58569-55-4 (Enkephalin, Methionine)
RN  - BBX060AN9V (Hydrogen Peroxide)
SB  - IM
MH  - Animals
MH  - Antibodies/pharmacology
MH  - Behavior, Animal/*drug effects
MH  - Edema/immunology
MH  - Emotions/drug effects/physiology
MH  - Enkephalin, Methionine/immunology/*pharmacology
MH  - Exploratory Behavior/drug effects/physiology
MH  - Hydrogen Peroxide/metabolism
MH  - Injections, Intraperitoneal
MH  - Injections, Intraventricular
MH  - Macrophages, Peritoneal/immunology/metabolism
MH  - Male
MH  - Neuroimmunomodulation/*drug effects
MH  - Nociceptors/drug effects/immunology
MH  - Rats
MH  - Rats, Wistar
EDAT- 2000/08/31 11:00
MHDA- 2000/10/14 11:01
CRDT- 2000/08/31 11:00
PHST- 2000/08/31 11:00 [pubmed]
PHST- 2000/10/14 11:01 [medline]
PHST- 2000/08/31 11:00 [entrez]
AID - 26455 [pii]
AID - 10.1159/000026455 [doi]
PST - ppublish
SO  - Neuroimmunomodulation. 2000;8(2):70-7. doi: 10.1159/000026455.