PMID- 10965519
OWN - NLM
STAT- MEDLINE
DCOM- 20010531
LR  - 20181130
IS  - 0163-5581 (Print)
IS  - 0163-5581 (Linking)
VI  - 37
IP  - 1
DP  - 2000
TI  - Genistein and curcumin block TGF-beta 1-induced u-PA expression and migratory and 
      invasive phenotype in mouse epidermal keratinocytes.
PG  - 49-54
AB  - Transforming growth factor-beta 1 (TGF-beta 1) stimulates migration/invasion of 
      mouse transformed keratinocytes and increases urokinase (u-PA) 
      expression/secretion. In this report, we analyzed the biological behavior of two 
      naturally occurring inhibitors of protein tyrosine kinases, genistein and 
      curcumin, that could abrogate the enhancement of u-PA levels induced by TGF-beta 
      1 in transformed keratinocytes. Our results showed that genistein and curcumin 
      blocked this response in a dose-dependent manner and also inhibited the TGF-beta 
      1-induced synthesis of fibronectin, an early responsive gene to the growth 
      factor. Both compounds also reduced TGF-beta 1-stimulated cell migration and 
      invasiveness. These results suggest that a tyrosine kinase-signaling pathway 
      should be involved in TGF-beta 1-mediated increased malignancy of transformed 
      keratinocytes and that genistein and curcumin could play an important role in 
      inhibiting tumor progression.
FAU - Santibanez, J F
AU  - Santibanez JF
AD  - Laboratorio de Biologia Celular, INTA, Universidad de Chile, Santiago, Chile.
FAU - Quintanilla, M
AU  - Quintanilla M
FAU - Martinez, J
AU  - Martinez J
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Nutr Cancer
JT  - Nutrition and cancer
JID - 7905040
RN  - 0 (Transforming Growth Factor beta)
RN  - DH2M523P0H (Genistein)
RN  - EC 2.7.10.1 (Protein-Tyrosine Kinases)
RN  - EC 3.4.21.73 (Urokinase-Type Plasminogen Activator)
RN  - IT942ZTH98 (Curcumin)
SB  - IM
MH  - Animals
MH  - Blotting, Western
MH  - Cell Movement/drug effects
MH  - Cells, Cultured
MH  - Curcumin/*pharmacology
MH  - Epidermal Cells
MH  - Epidermis/drug effects
MH  - Fluorescent Antibody Technique
MH  - Genistein/antagonists & inhibitors/*pharmacology
MH  - Keratinocytes/cytology/*drug effects/metabolism
MH  - Mice
MH  - Protein-Tyrosine Kinases/metabolism
MH  - Transforming Growth Factor beta/*pharmacology
MH  - Urokinase-Type Plasminogen Activator/*drug effects/metabolism
EDAT- 2000/08/31 11:00
MHDA- 2001/06/02 10:01
CRDT- 2000/08/31 11:00
PHST- 2000/08/31 11:00 [pubmed]
PHST- 2001/06/02 10:01 [medline]
PHST- 2000/08/31 11:00 [entrez]
AID - 10.1207/S15327914NC3701_6 [doi]
PST - ppublish
SO  - Nutr Cancer. 2000;37(1):49-54. doi: 10.1207/S15327914NC3701_6.