PMID- 10967052
OWN - NLM
STAT- MEDLINE
DCOM- 20000914
LR  - 20071114
IS  - 0146-0404 (Print)
IS  - 0146-0404 (Linking)
VI  - 41
IP  - 10
DP  - 2000 Sep
TI  - The combined effect of brain-derived neurotrophic factor and a free radical 
      scavenger in experimental glaucoma.
PG  - 2967-71
AB  - PURPOSE. Brain-derived neurotrophic factor (BDNF) had a limited effect on the 
      survival of retinal ganglion cells (RGCs) in rats' eyes with elevated intraocular 
      pressure (IOP). The combined treatment of BDNF and a nonspecific free radical 
      scavenger N-tert-butyl-(2-sulfophenyal)-nitrone (S-PBN) was investigated on the 
      RGCs in hypertensive eyes of rats. METHODS: Adult Wistar rats were separated into 
      five groups: BDNF (0.5 microg) + S-PBN; BDNF (1. 0 microg) + S-PBN; BDNF (1.0 
      microg); S-PBN; and phosphate-buffered saline. Right eyes served as normal 
      controls (n = 10). RGCs were labeled with 5% Fluoro Gold; injected into the 
      superior colliculus. Three days after intratectal injection, the episcleral veins 
      of the left eyes were cauterized. Intravitreal injection of BDNF was performed on 
      days 5, 13, 21, and 29 after IOP elevation. S-PBN was injected intraperitoneally 
      (100 mg/kg body wt) every 12 hours starting 30 minutes after cauterization. 
      RESULTS: The survival of RGCs using BDNF treatment alone in moderately 
      hypertensive eyes and systemic administration of S-PBN alone did not 
      significantly rescue the RGCs. However, the combination of BDNF and S-PBN 
      increased the survival of RGCs to 90.1%. CONCLUSIONS: Trophic factors and 
      antioxidants have synergistic effects on rescuing RGCs from death in eyes with 
      elevated IOP. Further studies of different combined treatment therapies may 
      provide avenues to save RGCs from death in eyes with elevated IOP.
FAU - Ko, M L
AU  - Ko ML
AD  - Department of Physiology, National Taiwan University, Taipei.
FAU - Hu, D N
AU  - Hu DN
FAU - Ritch, R
AU  - Ritch R
FAU - Sharma, S C
AU  - Sharma SC
LA  - eng
GR  - EY 11295/EY/NEI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Invest Ophthalmol Vis Sci
JT  - Investigative ophthalmology & visual science
JID - 7703701
RN  - 0 (Benzenesulfonates)
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Free Radical Scavengers)
RN  - 113443-50-8 (N-tert-butyl-(2-sulfophenyl)nitrone)
SB  - IM
MH  - Animals
MH  - Benzenesulfonates/*therapeutic use
MH  - Brain-Derived Neurotrophic Factor/*therapeutic use
MH  - Cell Count
MH  - Cell Survival
MH  - Disease Models, Animal
MH  - Drug Therapy, Combination
MH  - Female
MH  - Free Radical Scavengers/*therapeutic use
MH  - Injections, Intraperitoneal
MH  - Intraocular Pressure
MH  - Ocular Hypertension/*drug therapy/pathology
MH  - Rats
MH  - Rats, Wistar
MH  - Retinal Ganglion Cells/*drug effects/pathology
MH  - Vitreous Body
EDAT- 2000/09/01 11:00
MHDA- 2000/09/19 11:01
CRDT- 2000/09/01 11:00
PHST- 2000/09/01 11:00 [pubmed]
PHST- 2000/09/19 11:01 [medline]
PHST- 2000/09/01 11:00 [entrez]
PST - ppublish
SO  - Invest Ophthalmol Vis Sci. 2000 Sep;41(10):2967-71.