PMID- 10967399
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20191120
IS  - 1879-1514 (Electronic)
IS  - 0166-445X (Linking)
VI  - 50
IP  - 4
DP  - 2000 Oct 1
TI  - Induction of cytochrome P450 1A in the American Eel by model halogenated and 
      non-halogenated aryl hydrocarbon receptor agonists.
PG  - 375-386
AB  - Eels (Anguilla sp.) have phylogenetic, life history, and morphological 
      characteristics which distinguish them from many other species that have been 
      examined for cytochrome P450 1A (CYP1A) induction. Members of the family 
      Anguillidae often occur in regions of the coastal environment that are heavily 
      impacted by chemical contamination. Although eels have been suggested to be a 
      useful species for biomonitoring, the sensitivity with which eel CYP1A is induced 
      by aryl hydrocarbon receptor (AHR) agonists is not known. We investigated the 
      dose-dependent induction of hepatic CYP1A in the American eel (Anguilla 
      rostrata). Eels from an uncontaminated site were injected intra-peritoneally with 
      the model AHR agonists ss-naphthoflavone (BNF), benzo[a]pyrene (B[a]P) or 
      3,3',4,4-tetrachlorobiphenyl (TCB) at increasing doses (BNF at 0.1, 1, 10 and 100 
      mg/kg, B[a]P at 0.1, 1 and 10 mg/kg, and TCB at 0.1, 1, 10 and 20 mg/kg). All 
      three compounds produced dose-dependent induction of CYP1A content and catalytic 
      activity. An estimated ED(50) for induction of liver microsomal EROD activity by 
      TCB was approximately 5 mg/kg, indicating only moderate sensitivity. At 
      comparable doses of 1 and 10 mg/kg (or 3-4 and 30-40 micromol/kg), BNF and B[a]P had 
      2-3-fold greater effect than TCB in eliciting hepatic CYP1A induction. Injection 
      of radiolabeled B[a]P and TCB resulted in similar dose-dependent concentrations 
      of these compounds in eel liver, and the hepatic inducer concentrations and CYP1A 
      levels were correlated positively. Eels collected from New Bedford Harbor (NBH), 
      a Superfund site highly contaminated by polycyclic aromatic hydrocarbons and 
      polychlorinated biphenyls, had levels of microsomal CYP1A protein and EROD 
      activity that were equivalent to the highest levels induced experimentally. Eels 
      from less contaminated sites had correspondingly less CYP1A expression. The 
      responses to B[a]P or BNF as compared to TCB suggest a lower efficacy and/or 
      potency for CYP1A induction by TCB which could involve differences in the 
      mechanisms of responses to these compounds in eels. However, the moderate 
      sensitivity and the CYP1A induction in NBH eels support suggestions that eels may 
      be useful in monitoring more contaminated regions.
FAU - Schlezinger, JJ
AU  - Schlezinger JJ
AD  - Biology Department, Woods Hole Oceanographic Institution, Redfield 342, MS 32, 
      02543, Woods Hole, MA, USA
FAU - Stegeman, JJ
AU  - Stegeman JJ
LA  - eng
PT  - Journal Article
PL  - Netherlands
TA  - Aquat Toxicol
JT  - Aquatic toxicology (Amsterdam, Netherlands)
JID - 8500246
EDAT- 2000/09/01 00:00
MHDA- 2000/09/01 00:01
CRDT- 2000/09/01 00:00
PHST- 2000/09/01 00:00 [pubmed]
PHST- 2000/09/01 00:01 [medline]
PHST- 2000/09/01 00:00 [entrez]
AID - S0166445X00000874 [pii]
AID - 10.1016/s0166-445x(00)00087-4 [doi]
PST - ppublish
SO  - Aquat Toxicol. 2000 Oct 1;50(4):375-386. doi: 10.1016/s0166-445x(00)00087-4.