PMID- 10971214
OWN - NLM
STAT- MEDLINE
DCOM- 20001030
LR  - 20180213
IS  - 0042-9007 (Print)
IS  - 0042-9007 (Linking)
VI  - 79
IP  - 1
DP  - 2000
TI  - A clinically applicable method for the ex vivo generation of antigen-presenting 
      cells from CD34+ progenitors.
PG  - 46-52
AB  - BACKGROUND AND OBJECTIVES: Dendritic cells (DCs), the most potent of 
      antigen-presenting cells, can be generated in vitro from bone marrow or blood 
      progenitor cells. We have developed a method for producing such cells from 
      mobilised peripheral blood CD34+ cells in the absence of bovine products. 
      METHODS: The culture system developed used X-Vivo 10 culture medium with 10% 
      autologous serum, rhGM-CSF, rhTNF-alpha and rhIL-4. Large-scale cultures were 
      performed in Stericell 12 inch x 15 inch culture bags. RESULTS: In 12-small-scale 
      experiments, over 14 days, there was a median 38-fold increase in cell numbers of 
      which 12.8% were DCs as defined by immunophenotyping. These cells had potent DC 
      activity in functional assays. In two clinical-scale experiments, a 5.7- and 
      10-fold expansion of total cell numbers was obtained, with 8.2 and 18% of the 
      final population being DCs, respectively. CONCLUSION: This system is suitable for 
      clinical application.
FAU - Ardeshna, K M
AU  - Ardeshna KM
AD  - Department of Haematology, Royal Free and University College Medical School, 
      London, UK. k.ardeshna@ucl.ac.uk
FAU - Corney, C P
AU  - Corney CP
FAU - Ings, S J
AU  - Ings SJ
FAU - Watts, M J
AU  - Watts MJ
FAU - Linch, D C
AU  - Linch DC
FAU - Devereux, S
AU  - Devereux S
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Vox Sang
JT  - Vox sanguinis
JID - 0413606
RN  - 0 (Antigens, CD34)
SB  - IM
MH  - Animals
MH  - *Antigen Presentation
MH  - Antigens, CD34
MH  - Cattle
MH  - Cell Differentiation
MH  - Cell Lineage
MH  - Cells, Cultured
MH  - Dendritic Cells/*cytology/*immunology
MH  - Flow Cytometry
MH  - Hematopoietic Stem Cell Mobilization/methods
MH  - Hematopoietic Stem Cells/*cytology/*immunology
MH  - Humans
EDAT- 2000/09/06 11:00
MHDA- 2001/02/28 10:01
CRDT- 2000/09/06 11:00
PHST- 2000/09/06 11:00 [pubmed]
PHST- 2001/02/28 10:01 [medline]
PHST- 2000/09/06 11:00 [entrez]
AID - 31205 [pii]
AID - 10.1159/000031205 [doi]
PST - ppublish
SO  - Vox Sang. 2000;79(1):46-52. doi: 10.1159/000031205.