PMID- 10972973
OWN - NLM
STAT- MEDLINE
DCOM- 20010105
LR  - 20231213
IS  - 0730-2312 (Print)
IS  - 0730-2312 (Linking)
VI  - 79
IP  - 3
DP  - 2000 Sep 7
TI  - Growth inhibition in G(1) and altered expression of cyclin D1 and p27(kip-1 
      )after forced connexin expression in lung and liver carcinoma cells.
PG  - 347-54
AB  - Gap junctional intercellular communication (GJIC) and connexin expression are 
      frequently decreased in neoplasia and may contribute to defective growth control 
      and loss of differentiated functions. GJIC, in E9 mouse lung carcinoma cells and 
      WB-aB1 neoplastic rat liver epithelial cells, was elevated by forced expression 
      of the gap junction proteins, connexin43 (Cx43) and connexin32 (Cx32), 
      respectively. Transfection of Cx43 into E9 cells increased fluorescent 
      dye-coupling in the transfected clones, E9-2 and E9-3, to levels comparable to 
      the nontransformed sibling cell line, E10, from which E9 cells originated. 
      Transduction of Cx32 into WB-aB1 cells also increased dye-coupling in the clone, 
      WB-a/32-10, to a level that was comparable to the nontransformed sibling cell 
      line, WB-F344. The cell cycle distribution was also affected as a result of 
      forced connexin expression. The percentage of cells in G(1)-phase increased and 
      the percentage in S-phase decreased in E9-2 and WB-a/32-10 cells as compared to 
      E9 and WB-aB1 cells. Concomitantly, these cells exhibited changes in G(1)-phase 
      cell cycle regulators. E9-2 and WB-a/32-10 cells expressed significantly less 
      cyclin D1 and more p27(kip-1) protein than E9 and WB-aB1 cells. Other 
      growth-related properties (expression of platelet-derived growth factor 
      receptor-beta, epidermal growth factor receptor, protein kinase C-alpha, protein 
      kinase A regulatory subunit-Ialpha, and production of nitric oxide in response to 
      a cocktail of pro-inflammatory cytokines) were minimally altered or unaffected. 
      Thus, enhancement of connexin expression and GJIC in neoplastic mouse lung and 
      rat liver epithelial cells restored G(1) growth control. This was associated with 
      decreased expression of cyclin D1 and increased expression of p27(kip-1), but not 
      with changes in other growth-related functions.
CI  - Copyright 2000 Wiley-Liss, Inc.
FAU - Koffler, L
AU  - Koffler L
AD  - Department of Pathology, Medical College of Ohio, Toledo, Ohio 43699, USA.
FAU - Roshong, S
AU  - Roshong S
FAU - Kyu Park, I
AU  - Kyu Park I
FAU - Cesen-Cummings, K
AU  - Cesen-Cummings K
FAU - Thompson, D C
AU  - Thompson DC
FAU - Dwyer-Nield, L D
AU  - Dwyer-Nield LD
FAU - Rice, P
AU  - Rice P
FAU - Mamay, C
AU  - Mamay C
FAU - Malkinson, A M
AU  - Malkinson AM
FAU - Ruch, R J
AU  - Ruch RJ
LA  - eng
GR  - CA33497/CA/NCI NIH HHS/United States
GR  - CA57612/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Cell Biochem
JT  - Journal of cellular biochemistry
JID - 8205768
RN  - 0 (Cdkn1b protein, mouse)
RN  - 0 (Cdkn1b protein, rat)
RN  - 0 (Cell Cycle Proteins)
RN  - 0 (Connexins)
RN  - 0 (Cytokines)
RN  - 0 (Fluorescent Dyes)
RN  - 0 (Microtubule-Associated Proteins)
RN  - 0 (Neoplasm Proteins)
RN  - 0 (Receptors, Growth Factor)
RN  - 0 (Recombinant Fusion Proteins)
RN  - 0 (Tumor Suppressor Proteins)
RN  - 131461-61-5 (connexin 42)
RN  - 136601-57-5 (Cyclin D1)
RN  - 147604-94-2 (Cyclin-Dependent Kinase Inhibitor p27)
RN  - 31C4KY9ESH (Nitric Oxide)
RN  - EC 2.7.- (Protein Kinases)
SB  - IM
MH  - Animals
MH  - Carcinoma/*pathology
MH  - *Cell Communication
MH  - *Cell Cycle Proteins
MH  - Cell Division
MH  - Connexins/biosynthesis/genetics/*physiology
MH  - Cyclin D1/*biosynthesis/genetics
MH  - Cyclin-Dependent Kinase Inhibitor p27
MH  - Cytokines/pharmacology
MH  - Diffusion
MH  - Fluorescent Dyes/metabolism
MH  - G1 Phase/*physiology
MH  - Gap Junctions/*physiology
MH  - Gene Expression Regulation, Neoplastic/*drug effects
MH  - Liver Neoplasms, Experimental/*pathology
MH  - Lung Neoplasms/*pathology
MH  - Mice
MH  - Microtubule-Associated Proteins/*biosynthesis/genetics
MH  - Neoplasm Proteins/biosynthesis/genetics/*physiology
MH  - Nitric Oxide/biosynthesis
MH  - Protein Kinases/biosynthesis/genetics
MH  - Rats
MH  - Receptors, Growth Factor/biosynthesis/genetics
MH  - Recombinant Fusion Proteins/biosynthesis
MH  - Transfection
MH  - Tumor Cells, Cultured/drug effects/metabolism
MH  - *Tumor Suppressor Proteins
MH  - Gap Junction beta-1 Protein
EDAT- 2000/09/06 11:00
MHDA- 2001/02/28 10:01
CRDT- 2000/09/06 11:00
PHST- 2000/09/06 11:00 [pubmed]
PHST- 2001/02/28 10:01 [medline]
PHST- 2000/09/06 11:00 [entrez]
AID - 10.1002/1097-4644(20001201)79:3<347::AID-JCB10>3.0.CO;2-2 [pii]
AID - 10.1002/1097-4644(20001201)79:3<347::aid-jcb10>3.0.co;2-2 [doi]
PST - ppublish
SO  - J Cell Biochem. 2000 Sep 7;79(3):347-54. doi: 
      10.1002/1097-4644(20001201)79:3<347::aid-jcb10>3.0.co;2-2.