PMID- 10973462
OWN - NLM
STAT- MEDLINE
DCOM- 20001020
LR  - 20240509
IS  - 1071-412X (Print)
IS  - 1098-6588 (Electronic)
IS  - 1071-412X (Linking)
VI  - 7
IP  - 5
DP  - 2000 Sep
TI  - An immunoblot assay for detection of immunoglobulin M antibody to human 
      herpesvirus 6.
PG  - 823-7
AB  - We identified the human herpesvirus 6 (HHV-6)-dominant immunoglobulin M 
      (IgM)-reactive virion protein as being the same 101-kDa protein (101K) previously 
      identified as the major IgG immunoreactive protein and a specific serologic 
      marker of HHV-6 infection. An immunoblot assay (IB) to detect HHV-6-specific IgM 
      antibodies against the 101K protein in human serum samples was developed. The 
      assay was validated by using acute- and convalescent-phase serum collected from 
      children under 2 years of age in which we previously detected IgG seroconversion 
      to the HHV-6 101K protein. Of 32 serum pairs which previously demonstrated IgG 
      seroconversion to the 101K protein, 29 had IgM reactivity to the same protein in 
      the acute-phase sample and the remaining 3 had reactivity in the 
      convalescent-phase sample. We also detected HHV-6 IgM activity in sera collected 
      from individuals > or =4 years of age who were also IgM seropositive to measles 
      or rubella. Results of cross-adsorption studies using measles virus-, rubella 
      virus-, and HHV-6-infected cells as the adsorbing antigen indicated no 
      cross-reactivity between measles or rubella IgM and HHV-6 IgM in human serum 
      samples. The IgM IB detected HHV-6-specific IgM antibody to the 101K protein in 
      78% (63 of 81) of tested acute-phase serum collected from young children with an 
      undifferentiated rash illness by using a single serum dilution.
FAU - LaCroix, S
AU  - LaCroix S
AD  - State of Washington Public Health Laboratory, Seattle, Washington, USA.
FAU - Stewart, J A
AU  - Stewart JA
FAU - Thouless, M E
AU  - Thouless ME
FAU - Black, J B
AU  - Black JB
LA  - eng
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Clin Diagn Lab Immunol
JT  - Clinical and diagnostic laboratory immunology
JID - 9421292
RN  - 0 (101K protein, Human herpesvirus 6)
RN  - 0 (Antibodies, Viral)
RN  - 0 (Antigens, Viral)
RN  - 0 (Immunoglobulin M)
RN  - 0 (Viral Proteins)
SB  - IM
MH  - Antibodies, Viral/*blood/immunology
MH  - Antigens, Viral/*immunology
MH  - Child, Preschool
MH  - Cross Reactions
MH  - Herpesviridae Infections/blood/*immunology
MH  - Herpesvirus 6, Human/*immunology/isolation & purification
MH  - Humans
MH  - Immunoblotting/methods
MH  - Immunoglobulin M/*blood/immunology
MH  - Infant
MH  - Measles virus/immunology
MH  - Rubella virus/immunology
MH  - Viral Proteins/*immunology
PMC - PMC95963
EDAT- 2000/09/06 11:00
MHDA- 2001/02/28 10:01
PMCR- 2000/09/01
CRDT- 2000/09/06 11:00
PHST- 2000/09/06 11:00 [pubmed]
PHST- 2001/02/28 10:01 [medline]
PHST- 2000/09/06 11:00 [entrez]
PHST- 2000/09/01 00:00 [pmc-release]
AID - 0072 [pii]
AID - 10.1128/CDLI.7.5.823-827.2000 [doi]
PST - ppublish
SO  - Clin Diagn Lab Immunol. 2000 Sep;7(5):823-7. doi: 10.1128/CDLI.7.5.823-827.2000.