PMID- 10973691
OWN - NLM
STAT- MEDLINE
DCOM- 20001107
LR  - 20200505
IS  - 0165-2427 (Print)
IS  - 1873-2534 (Electronic)
IS  - 0165-2427 (Linking)
VI  - 76
IP  - 1-2
DP  - 2000 Aug 31
TI  - Effects of a porcine reproductive and respiratory syndrome virus infection on the 
      development of the immune response against pseudorabies virus.
PG  - 125-35
AB  - The aim of this study was to investigate the effects of a porcine reproductive 
      and respiratory syndrome virus (PRRSV) infection on the development of the immune 
      response after pseudorabies virus (PRV) vaccination in pigs. Pigs were 
      intranasally inoculated with the European PRRSV strain, Lelystad virus ter 
      Huurne, and were vaccinated intramuscularly with PRV 2 weeks later (LV-PRV 
      group). Control pigs were vaccinated with PRV only (PRV group). Eight weeks after 
      PRV vaccination, pigs from both groups were challenged intranasally with 
      wild-type PRV. We measured the lymphoproliferative, and the cytolytic responses 
      to PRV of peripheral blood mononuclear cells (PBMC), isolated from blood samples. 
      In addition, serum samples were examined for antibodies against PRV and LV. One 
      week after PRV vaccination, PBMC proliferated abundantly to PRV in both groups. 
      However, in the LV-PRV group the lymphoproliferative response declined after 1 
      week, whereas, in the PRV group, the lymphoproliferative response was high for 3 
      weeks and declined thereafter (P<0.05). After challenge, the lymphoproliferative 
      response was 1 week earlier and was consistently and significantly higher in the 
      PRV group than in the LV-PRV group. The PRV-specific killing was higher at 3 
      weeks after PRV vaccination and 5 weeks after PRV challenge 19+/-3 and 24+/-6%, 
      respectively, in the PRV group, compared to 7+/-4 and 6+/-9%, respectively, in 
      the LV-PRV group (P<0.05). However, later after vaccination and challenge the 
      cytolytic response was identical in both groups. The antibody titre against PRV 
      developed equally in both groups. After challenge, no PRV virus was isolated from 
      both groups. From these results we conclude that, although PRRSV infection did 
      cause changes in the time course of the T-lymphocyte response after PRV 
      vaccination, PRRSV infection did not inhibit the development of vaccine-induced 
      protection after PRV.
FAU - De Bruin, M G
AU  - De Bruin MG
AD  - Department of Mammalian Virology, Institute for Animal Science and Health 
      (ID-Lelystad), P.O. Box 65, 8200 AB Lelystad, The Netherlands. 
      m.g.m.debruin@id.wag-ur.nl
FAU - Samsom, J N
AU  - Samsom JN
FAU - Voermans, J J
AU  - Voermans JJ
FAU - van Rooij, E M
AU  - van Rooij EM
FAU - De Visser, Y E
AU  - De Visser YE
FAU - Bianchi, A T
AU  - Bianchi AT
LA  - eng
PT  - Journal Article
PL  - Netherlands
TA  - Vet Immunol Immunopathol
JT  - Veterinary immunology and immunopathology
JID - 8002006
RN  - 0 (Antibodies, Viral)
RN  - 0 (Viral Vaccines)
SB  - IM
EIN - Vet Immunol Immunopathol 2001 Jan 26;78(2):215-6
MH  - Administration, Intranasal
MH  - Animals
MH  - Antibodies, Viral/*biosynthesis
MH  - Cell Line
MH  - Cytotoxicity, Immunologic
MH  - Herpesvirus 1, Suid/*immunology
MH  - Immune Tolerance
MH  - Lymphocyte Activation
MH  - Porcine Reproductive and Respiratory Syndrome/*immunology
MH  - Porcine respiratory and reproductive syndrome virus
MH  - Swine
MH  - Swine, Miniature
MH  - Viral Vaccines/immunology
PMC - PMC7119737
EDAT- 2000/09/06 11:00
MHDA- 2001/02/28 10:01
PMCR- 2000/08/30
CRDT- 2000/09/06 11:00
PHST- 2000/09/06 11:00 [pubmed]
PHST- 2001/02/28 10:01 [medline]
PHST- 2000/09/06 11:00 [entrez]
PHST- 2000/08/30 00:00 [pmc-release]
AID - S0165-2427(00)00208-7 [pii]
AID - 10.1016/s0165-2427(00)00208-7 [doi]
PST - ppublish
SO  - Vet Immunol Immunopathol. 2000 Aug 31;76(1-2):125-35. doi: 
      10.1016/s0165-2427(00)00208-7.