PMID- 10973697
OWN - NLM
STAT- MEDLINE
DCOM- 20001122
LR  - 20201208
IS  - 0378-1135 (Print)
IS  - 0378-1135 (Linking)
VI  - 76
IP  - 3
DP  - 2000 Oct 1
TI  - Analysis of ORF 1 in European porcine reproductive and respiratory syndrome virus 
      by long RT-PCR and restriction fragment length polymorphism (RFLP) analysis.
PG  - 221-8
AB  - A rapid method was developed for partial characterization of the 
      replicase-encoding open reading frame 1 (ORF 1) of porcine reproductive and 
      respiratory syndrome virus (PRRSV). It comprised long RT-PCR amplification of 
      11.1 kb (94%) of ORF 1, followed by restriction fragment length polymorphism 
      analysis. The method was used to compare ORF 1 sequences of two divergent 
      European-type PRRSV strains. Our results indicated that the structural and 
      replicase parts of these two strains had evolved at overall similar rates.
FAU - Nielsen, H S
AU  - Nielsen HS
AD  - Danish Veterinary Institute for Virus Research, Ministry of Agriculture and 
      Fisheries, Lindholm, DK-4771 Kalvehave, Denmark.
FAU - Storgaard, T
AU  - Storgaard T
FAU - Oleksiewicz, M B
AU  - Oleksiewicz MB
LA  - eng
PT  - Journal Article
PL  - Netherlands
TA  - Vet Microbiol
JT  - Veterinary microbiology
JID - 7705469
RN  - 0 (DNA, Complementary)
RN  - 0 (RNA, Viral)
RN  - EC 2.7.7.48 (RNA-Dependent RNA Polymerase)
SB  - IM
MH  - Blotting, Southern/veterinary
MH  - Cloning, Molecular
MH  - DNA, Complementary/chemistry
MH  - *Open Reading Frames
MH  - Polymorphism, Restriction Fragment Length
MH  - Porcine respiratory and reproductive syndrome virus/*genetics
MH  - RNA, Viral/chemistry
MH  - RNA-Dependent RNA Polymerase/*metabolism
MH  - Reverse Transcriptase Polymerase Chain Reaction/*veterinary
EDAT- 2000/09/06 11:00
MHDA- 2001/02/28 10:01
CRDT- 2000/09/06 11:00
PHST- 2000/09/06 11:00 [pubmed]
PHST- 2001/02/28 10:01 [medline]
PHST- 2000/09/06 11:00 [entrez]
AID - S0378-1135(00)00258-3 [pii]
AID - 10.1016/s0378-1135(00)00258-3 [doi]
PST - ppublish
SO  - Vet Microbiol. 2000 Oct 1;76(3):221-8. doi: 10.1016/s0378-1135(00)00258-3.