PMID- 10974766
OWN - NLM
STAT- MEDLINE
DCOM- 20001107
LR  - 20061115
IS  - 0213-005X (Print)
IS  - 0213-005X (Linking)
VI  - 18
IP  - 5
DP  - 2000 May
TI  - [Detection of viral genomes of the Herpesviridae family in multiple sclerosis 
      patients by means of the polymerase chain reaction (PCR)].
PG  - 223-8
AB  - BACKGROUND: The multiple sclerosis seems to be the junction between genetics 
      alteration and an unknown environmental factor, that they would originate an 
      autoimmune alteration, that they would be the reason of the inflammation and 
      demyelinization responsible of the disease. Our objective has been the 
      determination of this possible environmental factor and to reach it, we have 
      studied the appearance of Herpesviridae family viruses. MATERIALS AND METHODS: 
      204 blood samples were studied: 102 from relapsing-remitting multiple sclerosis 
      patients (43 were undergoing beta-interferon treatment), and 102 from blood 
      donors with the same age and sex than multiple sclerosis patients. From this 
      samples, we extracted the DNA of peripheral blood mononuclear cells (PBMCs), and 
      we analyzed by polymerase chain reaction (PCR) to detect the appearance of herpes 
      simplex virus, varicella zoster virus, Epstein-Barr virus, cytomegalovirus, human 
      herpesvirus 6 (HHV-6), human herpesvirus 7 and human herpesvirus 8. RESULTS: a) 
      we only found significative difference (p = 0.0001) in HHV-6: 21.5% donors 
      positive samples (22/102), opposite to 49.02% of positivity in mulytiple 
      sclerosis patients (50/102); b) we didn't found significative differences in none 
      of other viruses studied, between patients treated with beta-interferon and 
      non-treated ones. CONCLUSIONS: Our results suggest us that HHV-6 can play an 
      important role in the multiple sclerosis development. The beta-interferon 
      treatment doesn't affect to DNA prevalence of none of studied viruses.
FAU - Alvarez, R
AU  - Alvarez R
AD  - Servicio de Microbiologia, Hospital Universitario San Carlos, Madrid.
FAU - Cour, I
AU  - Cour I
FAU - Kanaan, A
AU  - Kanaan A
FAU - Benedicto, M
AU  - Benedicto M
FAU - Martin-Estefania, C
AU  - Martin-Estefania C
FAU - Arroyo, R
AU  - Arroyo R
FAU - Varela de Seijas, E
AU  - Varela de Seijas E
FAU - Picazo, J J
AU  - Picazo JJ
LA  - spa
PT  - English Abstract
PT  - Journal Article
TT  - Determinacion de genomas de virus de la familia Herpesviridae en enfermos de 
      esclerosis multiple, por la reaccion en cadena de la polimerasa (PCR).
PL  - Spain
TA  - Enferm Infecc Microbiol Clin
JT  - Enfermedades infecciosas y microbiologia clinica
JID - 9104081
RN  - 0 (Antiviral Agents)
RN  - 0 (DNA, Viral)
RN  - 77238-31-4 (Interferon-beta)
SB  - IM
MH  - Adult
MH  - Antiviral Agents/therapeutic use
MH  - DNA, Viral/*blood
MH  - Female
MH  - *Genome, Viral
MH  - Herpesviridae/genetics/*isolation & purification
MH  - Herpesviridae Infections/complications/*epidemiology/virology
MH  - Herpesvirus 6, Human/genetics/isolation & purification/pathogenicity
MH  - Humans
MH  - Interferon-beta/therapeutic use
MH  - Lymphocytes/virology
MH  - Male
MH  - Middle Aged
MH  - Multiple Sclerosis/drug therapy/etiology/*virology
MH  - *Polymerase Chain Reaction/methods
MH  - Prevalence
MH  - Spain/epidemiology
MH  - Viremia/*virology
EDAT- 2000/09/07 11:00
MHDA- 2001/02/28 10:01
CRDT- 2000/09/07 11:00
PHST- 2000/09/07 11:00 [pubmed]
PHST- 2001/02/28 10:01 [medline]
PHST- 2000/09/07 11:00 [entrez]
PST - ppublish
SO  - Enferm Infecc Microbiol Clin. 2000 May;18(5):223-8.