PMID- 10975285
OWN - NLM
STAT- MEDLINE
DCOM- 20010125
LR  - 20100510
IS  - 0361-090X (Print)
IS  - 0361-090X (Linking)
VI  - 24
IP  - 3
DP  - 2000
TI  - Interleukin 18 induces a synergistic enhancement of interferon gamma production 
      in mixed murine spleen cell-tumor cell cultures: role of endogenous interleukin 
      12.
PG  - 234-43
AB  - Interleukin 18 (IL-18) reportedly synergizes with IL-12 and IL-10 for interferon 
      gamma (IFN-gamma) synthesis and natural killer (NK) cell activity, respectively. 
      Here we show that IL-18 alone induces low level IFN-gamma production by 
      unstimulated Balb/c mouse spleen cells, but production is enhanced 
      synergistically in cocultures of spleen cells and allogeneic living or fixed 
      Yac-1 cells. Spleen cells could be primed with IL-18 prior to coculture with 
      Yac-1 cells for IFN-gamma production, which also was observed in cocultures 
      containing either syngeneic or xenogeneic tumor cells. IFN-gamma production in 
      stimulated cocultures was abrogated almost completely by anti-IL-12 antibody and 
      was unrelated to spleen cell lytic activity. IL-10 moderately inhibited IFN-gamma 
      production induced by IL-18. Therefore, in spleen cell and tumor cell cocultures 
      exposed to IL-18, high levels of IFN-gamma are produced by the spleen cells 
      arising from a synergistic interaction between the exogenous IL-18 and endogenous 
      IL-12; however, this activity is unrelated to the spleen cell lytic activity.
FAU - Micallef, M J
AU  - Micallef MJ
AD  - Fujisaki Institute, Hayashibara Biochemical Laboratories, Okayama, Japan.
FAU - Tanimoto, T
AU  - Tanimoto T
FAU - Kohno, K
AU  - Kohno K
FAU - Ikegami, H
AU  - Ikegami H
FAU - Kurimoto, M
AU  - Kurimoto M
LA  - eng
PT  - Journal Article
PL  - England
TA  - Cancer Detect Prev
JT  - Cancer detection and prevention
JID - 7704778
RN  - 0 (Interleukin-18)
RN  - 130068-27-8 (Interleukin-10)
RN  - 187348-17-0 (Interleukin-12)
RN  - 82115-62-6 (Interferon-gamma)
SB  - IM
MH  - Animals
MH  - Cells, Cultured
MH  - Coculture Techniques
MH  - Dose-Response Relationship, Drug
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Female
MH  - Interferon-gamma/*biosynthesis
MH  - Interleukin-10/pharmacology
MH  - Interleukin-12/*physiology
MH  - Interleukin-18/pharmacology/*physiology
MH  - Killer Cells, Natural/metabolism
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Spleen/*metabolism
MH  - Time Factors
MH  - Tumor Cells, Cultured
EDAT- 2000/09/07 11:00
MHDA- 2001/02/28 10:01
CRDT- 2000/09/07 11:00
PHST- 2000/09/07 11:00 [pubmed]
PHST- 2001/02/28 10:01 [medline]
PHST- 2000/09/07 11:00 [entrez]
PST - ppublish
SO  - Cancer Detect Prev. 2000;24(3):234-43.