PMID- 10975523
OWN - NLM
STAT- MEDLINE
DCOM- 20000919
LR  - 20190705
IS  - 0092-8674 (Print)
IS  - 0092-8674 (Linking)
VI  - 102
IP  - 3
DP  - 2000 Aug 4
TI  - Developmental control of endocytosis in dendritic cells by Cdc42.
PG  - 325-34
AB  - Dendritic cells (DCs) developmentally regulate antigen uptake by controlling 
      their endocytic capacity. Immature DCs actively internalize antigen. However, 
      mature DCs are poorly endocytic, functioning instead to present antigens to T 
      cells. We have found that endocytic downregulation reflects a decrease in 
      endocytic activity controlled by Rho family GTPases, especially Cdc42. Blocking 
      Cdc42 function by Toxin B treatment or injection of dominant-negative inhibitors 
      of Cdc42 abrogates endocytosis in immature DCs. In mature DCs, injection of 
      constitutively active Cdc42 or microbial delivery of a Cdc42 nucleotide exchange 
      factor reactivates endocytosis. DCs regulate endogenous levels of Cdc42-GTP with 
      activated Cdc42 detectable only in immature cells. We conclude that DCs 
      developmentally regulate endocytosis at least in part by controlling levels of 
      activated Cdc42.
FAU - Garrett, W S
AU  - Garrett WS
AD  - Department of Cell Biology, Ludwig Institute for Cancer Research, Yale University 
      School of Medicine, New Haven, Connecticut 06520, USA.
FAU - Chen, L M
AU  - Chen LM
FAU - Kroschewski, R
AU  - Kroschewski R
FAU - Ebersold, M
AU  - Ebersold M
FAU - Turley, S
AU  - Turley S
FAU - Trombetta, S
AU  - Trombetta S
FAU - Galan, J E
AU  - Galan JE
FAU - Mellman, I
AU  - Mellman I
LA  - eng
GR  - AI-34098/AI/NIAID NIH HHS/United States
GR  - CA-46128/CA/NCI NIH HHS/United States
GR  - GM-29765/GM/NIGMS NIH HHS/United States
GR  - etc.
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Cell
JT  - Cell
JID - 0413066
RN  - 0 (Bacterial Proteins)
RN  - 0 (Bacterial Toxins)
RN  - 0 (Clathrin)
RN  - 0 (toxB protein, Clostridium difficile)
RN  - EC 3.6.5.2 (cdc42 GTP-Binding Protein)
SB  - IM
MH  - Animals
MH  - *Antigen Presentation
MH  - *Bacterial Proteins
MH  - Bacterial Toxins/pharmacology
MH  - Cell Differentiation
MH  - Clathrin
MH  - Coated Pits, Cell-Membrane
MH  - Dendritic Cells/*immunology
MH  - Down-Regulation
MH  - *Endocytosis
MH  - Enzyme Activation
MH  - Male
MH  - Mice
MH  - Pinocytosis
MH  - Salmonella typhimurium/immunology
MH  - cdc42 GTP-Binding Protein/antagonists & inhibitors/*metabolism
EDAT- 2000/09/07 11:00
MHDA- 2000/09/23 11:01
CRDT- 2000/09/07 11:00
PHST- 2000/09/07 11:00 [pubmed]
PHST- 2000/09/23 11:01 [medline]
PHST- 2000/09/07 11:00 [entrez]
AID - S0092-8674(00)00038-6 [pii]
AID - 10.1016/s0092-8674(00)00038-6 [doi]
PST - ppublish
SO  - Cell. 2000 Aug 4;102(3):325-34. doi: 10.1016/s0092-8674(00)00038-6.