PMID- 10976415
OWN - NLM
STAT- MEDLINE
DCOM- 20001229
LR  - 20190515
IS  - 1058-0468 (Print)
IS  - 1573-7330 (Electronic)
IS  - 1058-0468 (Linking)
VI  - 17
IP  - 5
DP  - 2000 May
TI  - FISH analysis of six chromosomes in unfertilized human oocytes after polar body 
      removal.
PG  - 276-83
AB  - PURPOSE: To develop an improved technique for estimating chromosomal 
      abnormalities in human oocytes by fluorescence in situ hybridization (FISH) and 
      to correlate the position of single chromatids with the chromosomal status of the 
      oocytes. METHODS: Oocytes that were at metaphase II about 17-20 hr after 
      insemination or intracytoplasmic sperm injection (ICSI) were treated with pronase 
      to remove the zona pellucida and polar body (PB) and then spread on slides using 
      HCl and Tween 20. Two rounds of FISH were performed using direct-labeled probes: 
      chromosomes 1, 13, 21 (round 1); chromosomes X, 7, 18 (round 2). RESULTS: Of the 
      63 oocytes from 18 patients (mean age, 32 years), 48 (76%) had one DNA complement 
      as expected, 9 (14%) had 2 DNA complements, 3 (5%) gave incomplete FISH signals, 
      and 3 (5%) were not analyzable. Of the 48 oocytes with one set of DNA, 48% were 
      haploid, 44% were aneuploid for one or more chromosomes, and 8% were polyploid. 
      We also found an increased frequency of predivision of chromatid bivalents in 
      aneuploid oocytes, especially for chromosome 21. CONCLUSIONS: This technique 
      enables simultaneous assessment of six chromosomes in human oocytes, and 
      therefore can be useful for accurately determining the incidence and causes of 
      genetic imbalances in human oocytes and apparently low fertilization rates.
FAU - Martini, E
AU  - Martini E
AD  - Department of Molecular Cell Biology and Genetics, University of Maastricht, The 
      Netherlands.
FAU - Flaherty, S P
AU  - Flaherty SP
FAU - Swann, N J
AU  - Swann NJ
FAU - Matthews, C D
AU  - Matthews CD
FAU - Ramaekers, F C
AU  - Ramaekers FC
FAU - Geraedts, J P
AU  - Geraedts JP
LA  - eng
PT  - Journal Article
PL  - Netherlands
TA  - J Assist Reprod Genet
JT  - Journal of assisted reproduction and genetics
JID - 9206495
RN  - 0 (DNA Probes)
RN  - 0 (Indoles)
RN  - 47165-04-8 (DAPI)
RN  - 9007-49-2 (DNA)
RN  - EC 3.4.24.- (Pronase)
SB  - IM
MH  - Adult
MH  - *Aneuploidy
MH  - *Cell Nucleus Structures
MH  - Chromatids/genetics/metabolism
MH  - DNA/analysis
MH  - DNA Probes
MH  - Female
MH  - Fertilization
MH  - Haploidy
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Indoles
MH  - Oocytes/*cytology/metabolism/*pathology
MH  - Polyploidy
MH  - Pronase/metabolism
MH  - Sperm Injections, Intracytoplasmic
PMC - PMC3455197
EDAT- 2000/09/08 11:00
MHDA- 2001/02/28 10:01
PMCR- 2001/05/01
CRDT- 2000/09/08 11:00
PHST- 2000/09/08 11:00 [pubmed]
PHST- 2001/02/28 10:01 [medline]
PHST- 2000/09/08 11:00 [entrez]
PHST- 2001/05/01 00:00 [pmc-release]
AID - 223633 [pii]
AID - 10.1023/a:1009462400708 [doi]
PST - ppublish
SO  - J Assist Reprod Genet. 2000 May;17(5):276-83. doi: 10.1023/a:1009462400708.