PMID- 10976738
OWN - NLM
STAT- MEDLINE
DCOM- 20010126
LR  - 20190915
IS  - 0002-9645 (Print)
IS  - 0002-9645 (Linking)
VI  - 61
IP  - 9
DP  - 2000 Sep
TI  - Concentrations of elastinolytic metalloproteinases in respiratory tract 
      secretions of healthy horses and horses with chronic obstructive pulmonary 
      disease.
PG  - 1067-73
AB  - OBJECTIVES: To determine whether samples of tracheal epithelial lining fluid 
      (TELF) obtained from horses have elastinolytic activity characteristic of 
      metalloproteinases, to compare elastinolytic activity in TELF obtained from 
      healthy horses and horses with chronic obstructive pulmonary disease (COPD), and 
      to determine whether chemically modified tetracycline-3 (CMT-3) inhibits 
      elastinolytic activity in TELF ANIMALS: 10 horses with COPD and 10 healthy 
      control horses. PROCEDURE: Zymography and fluorometry were used to measure 
      elastinolytic activity, and EDTA was used to inhibit elastinolytic activity and 
      verify that the activity was attributable to metalloproteinases. Possible 
      inhibition of elastinolytic activity with CMT-3 was studied in vitro. RESULTS: 
      Elastinolytic activity was found in TELF obtained from all horses, and this 
      activity was significantly higher in TELF obtained from horses with COPD than in 
      TELF obtained from healthy horses. For all samples, EDTA and CMT-3 inhibited 
      elastinolytic activity. CONCLUSIONS AND CLINICAL RELEVANCE: Elastinolytic 
      activity is detectable in TELF obtained from horses and seems to be attributable 
      to metalloproteinases. Elastinolytic activity in TELF is significantly inhibited 
      by CMT-3. Elastinolytic activity in TELF can be detected by means of zymography 
      or fluorometry. Increased elastinolytic activity may reflect destruction of 
      pulmonary tissue in horses with COPD. Chemically modified tetracyclines such as 
      CMT-3 may provide an additional treatment possibility for horses with COPD.
FAU - Raulo, S M
AU  - Raulo SM
AD  - Department of Clinical Veterinary Sciences, Faculty of Veterinary Medicine, 
      Helsinki University, Finland.
FAU - Sorsa, T A
AU  - Sorsa TA
FAU - Maisi, P S
AU  - Maisi PS
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Am J Vet Res
JT  - American journal of veterinary research
JID - 0375011
RN  - 0 (Protease Inhibitors)
RN  - 0 (Tetracyclines)
RN  - 0 (tetracycline CMT-3)
RN  - 9007-58-3 (Elastin)
RN  - 9G34HU7RV0 (Edetic Acid)
SB  - IM
MH  - Animals
MH  - Down-Regulation
MH  - Edetic Acid/pharmacology
MH  - Elastin/*metabolism
MH  - Female
MH  - Fluorometry/veterinary
MH  - Horse Diseases/*enzymology
MH  - Horses
MH  - Lung Diseases, Obstructive/enzymology/*veterinary
MH  - Male
MH  - Molecular Weight
MH  - Protease Inhibitors/pharmacology
MH  - Respiratory System/*enzymology
MH  - Tetracyclines/pharmacology
EDAT- 2000/09/08 11:00
MHDA- 2001/02/28 10:01
CRDT- 2000/09/08 11:00
PHST- 2000/09/08 11:00 [pubmed]
PHST- 2001/02/28 10:01 [medline]
PHST- 2000/09/08 11:00 [entrez]
AID - 10.2460/ajvr.2000.61.1067 [doi]
PST - ppublish
SO  - Am J Vet Res. 2000 Sep;61(9):1067-73. doi: 10.2460/ajvr.2000.61.1067.