PMID- 10978516
OWN - NLM
STAT- MEDLINE
DCOM- 20001207
LR  - 20190610
IS  - 0006-3002 (Print)
IS  - 0006-3002 (Linking)
VI  - 1493
IP  - 1-2
DP  - 2000 Sep 7
TI  - Identification of a negative regulatory DNA element for neuronal BC1 RNA 
      expression by RNA polymerase III.
PG  - 142-50
AB  - BC1 RNA is a neuronal cell-specific RNA polymerase III (Pol III) transcript. The 
      BC1 RNA gene has plural types of Pol III promoters, in addition to which an E-box 
      sequence (E2 site) acts as a transcriptional activator, which is recognized by a 
      brain-specific protein(s). Using an in vitro transcription system, we found that 
      the upstream region of the BC1 RNA gene contained a sequence that interfered with 
      the activity of the E-box element in a distance-independent manner. A tandem 
      repeat within this sequence, which was weakly homologous with the 
      neuron-restrictive silencer element (NRSE) found in the Pol II system, was 
      recognized by a brain nuclear protein. Consistently, the transcriptional activity 
      increased by deleting the tandem repeat sequence. We called this BC1 
      RNA-repressing element BCRE. The DNA-binding specificities of BCRE-binding 
      protein differed from that of NRSE-binding protein (NRSF). A similar protein with 
      an ability to bind to BCRE was also found in liver and kidney. Furthermore, the 
      glutamate analog kainic acid increased the DNA-binding of both E2 site-binding 
      protein and BCRE-binding protein, and then the levels of BC1 RNA also increased 
      transiently. Our results suggested that both positive and negative regulatory 
      elements contribute to neuronal BC1 RNA expression.
FAU - Kobayashi, S
AU  - Kobayashi S
AD  - Department of Biochemistry, College of Pharmacy, Nihon University, Narashinodai, 
      Funabashi, 274-8555, Chiba, Japan.
FAU - Kamo, S
AU  - Kamo S
FAU - Ohmae, A
AU  - Ohmae A
FAU - Agui, K
AU  - Agui K
FAU - Li, Y
AU  - Li Y
FAU - Anzai, K
AU  - Anzai K
LA  - eng
PT  - Journal Article
PL  - Netherlands
TA  - Biochim Biophys Acta
JT  - Biochimica et biophysica acta
JID - 0217513
RN  - 0 (BC1 RNA)
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (RNA, Small Cytoplasmic)
RN  - EC 2.7.7.6 (RNA Polymerase III)
RN  - SIV03811UC (Kainic Acid)
SB  - IM
MH  - Animals
MH  - Base Sequence
MH  - Brain/*metabolism
MH  - DNA-Binding Proteins/chemistry/*metabolism
MH  - Down-Regulation
MH  - Gene Expression Regulation
MH  - Kainic Acid
MH  - Male
MH  - Mice
MH  - Mice, Inbred ICR
MH  - Plasmids
MH  - RNA Polymerase III/genetics/*metabolism
MH  - RNA, Small Cytoplasmic/antagonists & inhibitors/*biosynthesis/genetics
MH  - Rats
MH  - Rats, Wistar
MH  - Seizures/chemically induced/metabolism
MH  - Transcription, Genetic
EDAT- 2000/09/09 11:00
MHDA- 2001/02/28 10:01
CRDT- 2000/09/09 11:00
PHST- 2000/09/09 11:00 [pubmed]
PHST- 2001/02/28 10:01 [medline]
PHST- 2000/09/09 11:00 [entrez]
AID - S0167-4781(00)00175-5 [pii]
AID - 10.1016/s0167-4781(00)00175-5 [doi]
PST - ppublish
SO  - Biochim Biophys Acta. 2000 Sep 7;1493(1-2):142-50. doi: 
      10.1016/s0167-4781(00)00175-5.