PMID- 10979967
OWN - NLM
STAT- MEDLINE
DCOM- 20001004
LR  - 20210216
IS  - 0006-4971 (Print)
IS  - 0006-4971 (Linking)
VI  - 96
IP  - 6
DP  - 2000 Sep 15
TI  - Identification of genes specifically expressed in human activated and mature 
      dendritic cells through serial analysis of gene expression.
PG  - 2206-14
AB  - Dendritic cells (DCs) are professional antigen-presenting cells in the immune 
      system and can be generated in vitro from hematopoietic progenitor cells, DC 
      precursors, and monocytes in peripheral blood. Serial analysis of gene expression 
      (SAGE) was conducted in lipopolysaccharide (LPS)-stimulated mature and activated 
      DCs (MADCs) derived from human blood monocytes. A total of 31 837 tag sequences 
      from an MADC cDNA library represented 10 962 different genes, and these data were 
      compared with SAGE data for monocyte-derived immature DCs (IMDCs). Many of the 
      genes, such as germinal center kinase-related protein kinase, cystatin F, 
      interferon (IFN)-alpha-inducible protein p27, EBI3, HEM45, actin-bundling 
      protein, ELC, DC-LAMP, serine/threonine kinase 4, and several genes in expressed 
      sequence tags, were differentially expressed in MADCs, and those encode proteins 
      related to cell structure, antigen-processing enzymes, chemokines, and 
      IFN-inducible proteins. The profile of MADCs was also compared with that of 
      LPS-stimulated monocytes. The Epstein-Barr virus-induced gene 3 and 
      IFN-alpha-inducible protein p27 are newly identified to be specifically and 
      highly expressed in MADCs, but not in LPS-stimulated monocytes. The comprehensive 
      identification of specific genes expressed in human IMDCs and MADCs should 
      provide candidate genes to define heterogeneous subsets as well as the function 
      and maturation stage of DCs.
FAU - Hashimoto, S I
AU  - Hashimoto SI
AD  - Department of Molecular Preventive Medicine and CREST, School of Medicine, 
      University of Tokyo, Tokyo, Japan.
FAU - Suzuki, T
AU  - Suzuki T
FAU - Nagai, S
AU  - Nagai S
FAU - Yamashita, T
AU  - Yamashita T
FAU - Toyoda, N
AU  - Toyoda N
FAU - Matsushima, K
AU  - Matsushima K
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Blood
JT  - Blood
JID - 7603509
SB  - IM
MH  - Antigen Presentation/*genetics
MH  - Cell Differentiation/physiology
MH  - Dendritic Cells/cytology/*physiology
MH  - Gene Expression Profiling
MH  - Gene Expression Regulation/*immunology
MH  - Humans
MH  - Multigene Family
EDAT- 2000/09/09 11:00
MHDA- 2000/10/07 11:01
CRDT- 2000/09/09 11:00
PHST- 2000/09/09 11:00 [pubmed]
PHST- 2000/10/07 11:01 [medline]
PHST- 2000/09/09 11:00 [entrez]
AID - S0006-4971(20)54410-8 [pii]
PST - ppublish
SO  - Blood. 2000 Sep 15;96(6):2206-14.