PMID- 10979968
OWN - NLM
STAT- MEDLINE
DCOM- 20001004
LR  - 20210216
IS  - 0006-4971 (Print)
IS  - 0006-4971 (Linking)
VI  - 96
IP  - 6
DP  - 2000 Sep 15
TI  - Detection of t(11;18)(q21;q21) by interphase fluorescence in situ hybridization 
      using API2 and MLT specific probes.
PG  - 2215-8
AB  - The translocation of chromosome 11, long arm, region 2, band 1, to chromosome 18, 
      long arm, region 2, band 1 (t(11;18)(q21;q21)) represents a recurrent chromosomal 
      abnormality in extranodal marginal zone B-cell lymphoma (MZBCL) of 
      mucosa-associated lymphoid tissue (MALT) type and leads to a fusion of the 
      apoptosis inhibitor-2 (API2) gene on chromosome 11 and the MALT 
      lymphoma-associated translocation (MLT) gene on chromosome 18. A 2-color 
      fluorescence in situ hybridization (FISH) assay, which can be used for the 
      detection of t(11;18) in interphase nuclei and metaphase chromosomes on fresh and 
      archival tumor tissue, was developed. The P1 artificial chromosome (PAC) clone 
      located immediately telomeric to the MLT gene and the PAC clone spanning the API2 
      gene were differentially labeled and used to visualize the derivative chromosome 
      11 resulting from t(11;18), as evident by the overlapping or juxtaposed red and 
      green fluorescent signals. The assay was applied to interphase nuclei of 20 cases 
      with nonmalignant conditions and 122 B-cell non-Hodgkin's lymphomas (NHLs). The 
      latter group comprised 20 cases of nodal follicle center cell lymphoma and 
      diffuse large B-cell NHL, 10 cases of gastric diffuse large B-cell lymphoma, 10 
      cases of hairy cell leukemia, and 82 cases of MZBCL (41 extranodal from various 
      locations, 19 nodal, and 22 splenic MZBCL) including 35 cases with an abnormal 
      karyotype, 2 of which revealed t(11;18). By interphase FISH, t(11;18) was 
      detected in 8 gastrointestinal low-grade MALT-type lymphomas including the 2 
      cytogenetically t(11;18)(+) cases. In the 8 t(11;18)(+) cases, the FISH results 
      were confirmed by reverse transcriptase-polymerase chain reaction (RT-PCR) using 
      API2 and MLT specific primers. Our results indicate that t(11;18)(q21;q21) 
      specifically characterizes a subgroup of low-grade MZBCL of the MALT-type and 
      that the FISH assay described here is a highly specific and rapid test for the 
      detection of this translocation.
FAU - Dierlamm, J
AU  - Dierlamm J
AD  - Department of Oncology and Hematology, University Hospital Eppendorf, Hamburg, 
      Germany.
FAU - Baens, M
AU  - Baens M
FAU - Stefanova-Ouzounova, M
AU  - Stefanova-Ouzounova M
FAU - Hinz, K
AU  - Hinz K
FAU - Wlodarska, I
AU  - Wlodarska I
FAU - Maes, B
AU  - Maes B
FAU - Steyls, A
AU  - Steyls A
FAU - Driessen, A
AU  - Driessen A
FAU - Verhoef, G
AU  - Verhoef G
FAU - Gaulard, P
AU  - Gaulard P
FAU - Hagemeijer, A
AU  - Hagemeijer A
FAU - Hossfeld, D K
AU  - Hossfeld DK
FAU - De Wolf-Peeters, C
AU  - De Wolf-Peeters C
FAU - Marynen, P
AU  - Marynen P
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Blood
JT  - Blood
JID - 7603509
RN  - 0 (Inhibitor of Apoptosis Proteins)
RN  - 0 (Neoplasm Proteins)
RN  - 0 (Oligonucleotide Probes)
RN  - 0 (Proteins)
RN  - EC 3.4.22.- (Caspases)
RN  - EC 3.4.22.- (MALT1 protein, human)
RN  - EC 3.4.22.- (Mucosa-Associated Lymphoid Tissue Lymphoma Translocation 1 Protein)
SB  - IM
MH  - Caspases
MH  - *Chromosomes, Human, Pair 11
MH  - *Chromosomes, Human, Pair 18
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Inhibitor of Apoptosis Proteins
MH  - Interphase
MH  - Lymphoma, B-Cell, Marginal Zone/*genetics
MH  - Mucosa-Associated Lymphoid Tissue Lymphoma Translocation 1 Protein
MH  - *Neoplasm Proteins
MH  - Oligonucleotide Probes
MH  - Proteins/genetics
MH  - *Translocation, Genetic
EDAT- 2000/09/09 11:00
MHDA- 2000/10/07 11:01
CRDT- 2000/09/09 11:00
PHST- 2000/09/09 11:00 [pubmed]
PHST- 2000/10/07 11:01 [medline]
PHST- 2000/09/09 11:00 [entrez]
AID - S0006-4971(20)54411-X [pii]
PST - ppublish
SO  - Blood. 2000 Sep 15;96(6):2215-8.