PMID- 10981527
OWN - NLM
STAT- MEDLINE
DCOM- 20010126
LR  - 20191104
IS  - 0905-6157 (Print)
IS  - 0905-6157 (Linking)
VI  - 11
IP  - 3
DP  - 2000 Aug
TI  - Expression of and responses to CD2 and CD3 in 18-month-old children with and 
      without atopic dermatitis.
PG  - 175-82
AB  - We hypothesize that atopy is associated with a reduced T-cell function early in 
      life and an imbalance in cytokine production. The purpose of this study was to 
      investigate the expression of and responses to CD2 and CD3 in children who did or 
      did not develop atopic dermatitis early in life. The expression of CD2 and CD3 
      was analyzed by flow cytometry, and proliferation of CD2 and CD3 was studied by 
      3H-thymidine incorporation in phytohaemagglutinin (PHA)- and anti-CD3-stimulated 
      peripheral blood mononuclear cells (PBMC) of 18-month-old children, 25 with and 
      29 without atopic dermatitis. Exogenous interleukin (IL)-2 was added to 
      compensate for possible functional differences in accessory cells. 
      Anti-CD3-induced secretion of IL-4, IL-5, IL-6, IL-10, IL-13, and 
      interferon-gamma (IFN-gamma) was analyzed by enzyme-linked immunosorbent assay 
      (ELISA). Atopy was associated with a low proportion of CD2+ lymphocytes. 
      Responsiveness to PHA, which activates lymphocytes partly via the sheep 
      erythrocyte receptor, CD2, was reduced in the allergic children. The 
      anti-CD3-induced proliferation declined more rapidly with antibody dilution in 
      the allergic than in the non-allergic children. Atopic dermatitis was associated 
      with high levels of anti-CD3-stimulated IL-5 secretion. The IL-4/IL-10 and 
      IL-4/ITFN-gamma ratios were higher in children with elevated total immunoglobulin 
      E (IgE) levels. Skin prick test-negative children with eczema produced higher 
      levels of IL-10 than skin prick test-positive children. In conclusion, atopic 
      children have a reduced T-cell function. Atopic dermatitis is associated with 
      increased IL-5 production, while high total IgE levels are associated with high 
      IL-4/IFN-gamma and IL-4/IL-10 ratios.
FAU - Jenmalm, M C
AU  - Jenmalm MC
AD  - Department of Health and Environment, Clinical Research Center, Faculty of Health 
      Sciences, Linkoping University, Sweden. Maria.Jenmalm@kfc.liu.se
FAU - Aniansson-Zdolsek, H
AU  - Aniansson-Zdolsek H
FAU - Holt, P G
AU  - Holt PG
FAU - Bjorksten, B
AU  - Bjorksten B
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Pediatr Allergy Immunol
JT  - Pediatric allergy and immunology : official publication of the European Society 
      of Pediatric Allergy and Immunology
JID - 9106718
RN  - 0 (CD2 Antigens)
RN  - 0 (CD3 Complex)
RN  - 0 (Interleukin-13)
RN  - 0 (Interleukin-5)
RN  - 0 (Interleukin-6)
RN  - 130068-27-8 (Interleukin-10)
RN  - 207137-56-2 (Interleukin-4)
RN  - 82115-62-6 (Interferon-gamma)
SB  - IM
MH  - CD2 Antigens/*biosynthesis/immunology
MH  - CD3 Complex/*biosynthesis/immunology
MH  - Dermatitis, Atopic/*immunology
MH  - Female
MH  - Humans
MH  - Infant
MH  - Interferon-gamma/biosynthesis
MH  - Interleukin-10/biosynthesis
MH  - Interleukin-13/biosynthesis
MH  - Interleukin-4/biosynthesis
MH  - Interleukin-5/biosynthesis
MH  - Interleukin-6/biosynthesis
MH  - Male
MH  - Surveys and Questionnaires
MH  - T-Lymphocytes/*immunology
EDAT- 2000/09/12 11:00
MHDA- 2001/02/28 10:01
CRDT- 2000/09/12 11:00
PHST- 2000/09/12 11:00 [pubmed]
PHST- 2001/02/28 10:01 [medline]
PHST- 2000/09/12 11:00 [entrez]
AID - 10.1034/j.1399-3038.2000.00083.x [doi]
PST - ppublish
SO  - Pediatr Allergy Immunol. 2000 Aug;11(3):175-82. doi: 
      10.1034/j.1399-3038.2000.00083.x.