PMID- 10982031
OWN - NLM
STAT- MEDLINE
DCOM- 20000921
LR  - 20190722
IS  - 0340-6717 (Print)
IS  - 0340-6717 (Linking)
VI  - 107
IP  - 1
DP  - 2000 Jul
TI  - Female fetal cells in maternal blood: use of DNA polymorphisms to prove origin.
PG  - 28-32
AB  - The nucleated erythrocyte (NRBC) is one of the target fetal cell types for 
      noninvasive genetic diagnosis using maternal peripheral blood. However, it is now 
      known that pregnancy can stimulate the production of maternal NRBCs. When 
      isolating female gamma-positive NRBCs, fluorescence in situ hybridization (FISH) 
      analysis may show two X chromosome signals per nucleus, and therefore it cannot 
      be conclusively determined whether the isolated cells are fetal or maternal in 
      origin. The purpose of this study was to develop a means of verifying that a 
      female cell is fetal on the basis of polymorphic short tandem repeat markers. 
      Peripheral blood samples were obtained from women who had just undergone 
      termination of pregnancy. Nucleated candidate fetal cells were isolated by 
      flow-sorting using antibody to the gamma-chain of fetal hemoglobin and Hoechst 
      33342. FISH analysis was performed using X and Y chromosome specific probes. 
      Female gamma-positive cells and leukocytes were micromanipulated separately and 
      subjected to fluorescent polymerase chain reaction amplification of chromosome 21 
      and/or 18 STR markers (D21S11, D21S1411, D21S1412, and D18S535). In all ten cases 
      analyzed, the gamma-positive female candidate fetal cells were determined to be 
      fetal in origin by the presence of shared and nonshared DNA polymorphisms when 
      compared with maternal leukocytes. These results show that genetic analysis can 
      be performed on all fetal NRBCs, including female fetal cells that cannot be 
      distinguished from maternal cells based on FISH analysis alone.
FAU - Samura, O
AU  - Samura O
AD  - Department of Pediatrics, Obstetrics and Gynecology, New England Medical Center 
      and Tufts University School of Medicine, Boston, MA 02111, USA.
FAU - Pertl, B
AU  - Pertl B
FAU - Sohda, S
AU  - Sohda S
FAU - Johnson, K L
AU  - Johnson KL
FAU - Sekizawa, A
AU  - Sekizawa A
FAU - Falco, V M
AU  - Falco VM
FAU - Elmes, R S
AU  - Elmes RS
FAU - Bianchi, D W
AU  - Bianchi DW
LA  - eng
GR  - N-HD4-3204/HD/NICHD NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - Germany
TA  - Hum Genet
JT  - Human genetics
JID - 7613873
RN  - 0 (Fluorescent Dyes)
RN  - 0 (Genetic Markers)
RN  - 9007-49-2 (DNA)
SB  - IM
MH  - Cell Separation
MH  - Chromosomes, Human, Pair 21
MH  - DNA/*analysis/*blood
MH  - Erythroblasts/*chemistry
MH  - Female
MH  - Fetus/*cytology
MH  - Fluorescent Dyes/metabolism
MH  - Genetic Markers
MH  - Gestational Age
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Polymerase Chain Reaction
MH  - *Polymorphism, Genetic
MH  - Pregnancy
MH  - *Tandem Repeat Sequences
EDAT- 2000/09/12 11:00
MHDA- 2000/09/23 11:01
CRDT- 2000/09/12 11:00
PHST- 2000/09/12 11:00 [pubmed]
PHST- 2000/09/23 11:01 [medline]
PHST- 2000/09/12 11:00 [entrez]
AID - 10.1007/s004390000327 [doi]
PST - ppublish
SO  - Hum Genet. 2000 Jul;107(1):28-32. doi: 10.1007/s004390000327.