PMID- 10982282
OWN - NLM
STAT- MEDLINE
DCOM- 20010125
LR  - 20061115
IS  - 0268-3369 (Print)
IS  - 0268-3369 (Linking)
VI  - 26
IP  - 4
DP  - 2000 Aug
TI  - Dendritic cells can be successfully generated from CD34+ cord blood cells in the 
      presence of autologous cord blood plasma.
PG  - 371-6
AB  - Dendritic cells (DCs) are currently being considered as adjuvants in 
      immunotherapy. Depending on their source and culture conditions, they show 
      different features and maturation states. Dendritic cells can be generated from 
      monocytes and CD34+ haematopoietic stem cells, from both adult and cord blood. 
      Here, we report the generation of mature DCs from enriched CD34+ cord blood (CB) 
      cells using autologous cord blood plasma (ACBP) as a source of serum proteins and 
      factors. In the presence of ACBP, CD34+ cells proliferated and differentiated 
      resulting in a population of cells with a dendritic phenotype as assessed by 
      morphology and flow cytometry analyses. The DC population obtained using ACBP 
      showed higher levels of HLA class II molecules, co-stimulatory molecules 
      including CD40, CD80 or CD86, and the dendritic cell marker CD83, compared with 
      those generated in adult blood serum (ABS). Furthermore, the DCs generated in the 
      presence of ACBP were more potent stimulatory cells in the mixed 
      lymphocyte:dendritic cell reactions (MLDCR), compared to cells generated in ABS. 
      Similar results were obtained using homologous cord blood plasma (HCBP). These 
      results show that ACBP can support the generation of DCs from CD34+ progenitor 
      cells when only GM-CSF and TNFalpha are used as differentiating cytokines.
FAU - Borras, F E
AU  - Borras FE
AD  - Department of Histocompatibility and Immunogenetics, NBS, North London Centre, 
      London, UK.
FAU - Matthews, N C
AU  - Matthews NC
FAU - Patel, R
AU  - Patel R
FAU - Navarrete, C
AU  - Navarrete C
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PL  - England
TA  - Bone Marrow Transplant
JT  - Bone marrow transplantation
JID - 8702459
RN  - 0 (Antigens, CD34)
RN  - 0 (Culture Media)
RN  - 0 (HLA-DQ Antigens)
RN  - 0 (HLA-DR Antigens)
SB  - IM
MH  - Adult
MH  - Antigens, CD34/*blood
MH  - Cell Culture Techniques/methods
MH  - Cell Differentiation/drug effects
MH  - Cell Division/drug effects
MH  - Culture Media/pharmacology
MH  - Dendritic Cells/*cytology/immunology
MH  - Fetal Blood/*cytology/immunology
MH  - HLA-DQ Antigens/blood/drug effects
MH  - HLA-DR Antigens/blood/drug effects
MH  - Humans
MH  - Immunophenotyping
MH  - Lymphocyte Culture Test, Mixed
EDAT- 2000/09/12 11:00
MHDA- 2001/02/28 10:01
CRDT- 2000/09/12 11:00
PHST- 2000/09/12 11:00 [pubmed]
PHST- 2001/02/28 10:01 [medline]
PHST- 2000/09/12 11:00 [entrez]
AID - 10.1038/sj.bmt.1702525 [doi]
PST - ppublish
SO  - Bone Marrow Transplant. 2000 Aug;26(4):371-6. doi: 10.1038/sj.bmt.1702525.