PMID- 10988316
OWN - NLM
STAT- MEDLINE
DCOM- 20001010
LR  - 20190727
IS  - 0041-1132 (Print)
IS  - 0041-1132 (Linking)
VI  - 40
IP  - 9
DP  - 2000 Sep
TI  - Cytokines in WBC-reduced apheresis PCs during storage: a comparison of two 
      WBC-reduction methods.
PG  - 1118-26
AB  - BACKGROUND: Several studies have suggested that cytokine accumulation during 
      storage of platelet concentrates (PCs) may mediate nonhemolytic febrile 
      transfusion reactions and that a reduction in WBC numbers prevents the generation 
      of cytokines. Despite efforts to minimize WBC contamination in apheresis PCs, 
      high numbers of WBCs and increased cytokine levels may still occur, depending on 
      the quality of the apheresis device employed. STUDY DESIGN AND METHODS: This 
      study was undertaken to investigate whether PCs collected with WBC-reduction 
      devices (Spectra LRS, COBE;or MCS+ LDP, Haemonetics) were sufficiently depleted 
      of WBCs to limit cytokine accumulation during storage. The study evaluated 1) the 
      levels of cytokines of WBC and platelet origin in two types of apheresis PCs 
      during storage and 2) the effects of prestorage filtration on cytokine levels in 
      the Spectra LRS PCs. RESULTS: In the Spectra LRS PCs, low levels of IL-6, IL-8, 
      and monotype chemoattractant protein 1 (MCP-1) were detected in Day 1 PCs, and 
      they remained consistent during the shelf life. RANTES, platelet factor 4 (PF4), 
      beta-thromboglobulin (beta-TG), and transforming growth factor (TGF)-beta1 were 
      also detected in these PCs, and their levels increased significantly on storage. 
      Prestorage filtration of Spectra LRS PCs did not further reduce the levels of 
      IL-6, IL-8, MCP-1, PF4, beta-TG, and TGF-beta1 in the filtered component. In the 
      MCS+ LDP PCs, IL-6 was detected on Day 1, and its level increased significantly 
      on storage, whereas the levels in the Spectra PCs remained steady. IL-8 levels 
      were lower in MCS+ LDP PCs than in Spectra LRS PCs of the same age. MCP-1 levels 
      were similar in both products on Day 1 and marginally increased in stored MCS+ 
      LDP PCs. Substantial amounts of RANTES, PF4, beta-TG, and TGF-beta1 occurred in 
      Day 1 MCS+ LDP PCs, and, on storage, these levels rose significantly. CONCLUSION: 
      Despite a significant reduction in levels of WBC-derived cytokines, 
      platelet-derived cytokines were present in different amounts in the two products.
FAU - Wadhwa, M
AU  - Wadhwa M
AD  - Divisions of Immunobiology and Informatics, National Institute for Biological 
      Standards and Control, Potters Bar, United Kingdom. mwadhwa@nibsc.ac.uk
FAU - Seghatchian, M J
AU  - Seghatchian MJ
FAU - Dilger, P
AU  - Dilger P
FAU - Sands, D
AU  - Sands D
FAU - Krailadisiri, P
AU  - Krailadisiri P
FAU - Contreras, M
AU  - Contreras M
FAU - Thorpe, R
AU  - Thorpe R
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PL  - United States
TA  - Transfusion
JT  - Transfusion
JID - 0417360
RN  - 0 (Chemokine CCL2)
RN  - 0 (Chemokine CCL5)
RN  - 0 (Cytokines)
RN  - 0 (Interleukin-6)
RN  - 0 (Interleukin-8)
RN  - 0 (Transforming Growth Factor beta)
RN  - 0 (beta-Thromboglobulin)
RN  - 37270-94-3 (Platelet Factor 4)
SB  - IM
MH  - Blood Platelets/*cytology
MH  - *Blood Preservation
MH  - Chemokine CCL2/blood
MH  - Chemokine CCL5/blood
MH  - Cytokines/*blood
MH  - Filtration
MH  - Humans
MH  - Interleukin-6/blood
MH  - Interleukin-8/blood
MH  - Leukapheresis/*methods
MH  - Platelet Factor 4/analysis
MH  - Time Factors
MH  - Transforming Growth Factor beta/blood
MH  - beta-Thromboglobulin/analysis
EDAT- 2000/09/16 11:00
MHDA- 2000/10/14 11:01
CRDT- 2000/09/16 11:00
PHST- 2000/09/16 11:00 [pubmed]
PHST- 2000/10/14 11:01 [medline]
PHST- 2000/09/16 11:00 [entrez]
AID - 10.1046/j.1537-2995.2000.40091118.x [doi]
PST - ppublish
SO  - Transfusion. 2000 Sep;40(9):1118-26. doi: 10.1046/j.1537-2995.2000.40091118.x.