PMID- 10989961
OWN - NLM
STAT- MEDLINE
DCOM- 20010104
LR  - 20041117
IS  - 0753-3322 (Print)
IS  - 0753-3322 (Linking)
VI  - 54
IP  - 6
DP  - 2000 Jul
TI  - Dysfunctional regulation of the development of monocyte-derived dendritic cells 
      in cancer patients.
PG  - 291-8
AB  - Dendritic cells (DCs) are highly effective antigen (Ag)-presenting cells (APCs) 
      that are required for the initiation of the immune response. DCs derived from 
      cancer patients have been shown to be defective in several phenotypic and 
      functional properties. However, little is known about the capacity of monocytes 
      derived from cancer patients to differentiate into DCs. Herein, we examined the 
      differentiation of monocyte-derived DCs in cancer patients. Flow cytometric 
      analysis revealed that monocytes derived from cancer patients cultured with 
      granulocyte-macrophage colony-stimulating factor (GM-CSF) plus interleukin-4 
      (IL-4) exhibited lower levels of CD11c, CD40, CD86, and HLA-DR expression as 
      compared with those of monocyte-derived DCs from healthy volunteers. Furthermore, 
      the capacities of DCs derived from cancer patients' monocytes to stimulate 
      allogeneic T cell responses and to migrate in response to regulated-on-activation 
      normal T cells expressed and secreted (RANTES) were impaired in comparison with 
      those of monocyte-derived DCs from healthy volunteers. However, the two cell 
      types had similar pinocytotic capacities for fluorescein isothiocyanate 
      labeled-dextran (FITC-DX) and lucifer yellow (LY). These results suggest that 
      monocytes from cancer patients may be defective in the capacity to develop into 
      DCs.
FAU - Hasebe, H
AU  - Hasebe H
AD  - Department of Surgery, The Institute of Medical Science. The University of Tokyo, 
      Japan.
FAU - Nagayama, H
AU  - Nagayama H
FAU - Sato, K
AU  - Sato K
FAU - Enomoto, M
AU  - Enomoto M
FAU - Takeda, Y
AU  - Takeda Y
FAU - Takahashi, T A
AU  - Takahashi TA
FAU - Hasumi, K
AU  - Hasumi K
FAU - Eriguchi, M
AU  - Eriguchi M
LA  - eng
PT  - Journal Article
PL  - France
TA  - Biomed Pharmacother
JT  - Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie
JID - 8213295
RN  - 0 (Antigens, CD)
RN  - 0 (HLA-DR Antigens)
SB  - IM
MH  - Aged
MH  - Antigens, CD/analysis
MH  - Cell Differentiation
MH  - Dendritic Cells/*physiology
MH  - Endocytosis
MH  - Female
MH  - HLA-DR Antigens/analysis
MH  - Humans
MH  - Male
MH  - Middle Aged
MH  - Monocytes/*physiology
MH  - Neoplasms/*immunology
MH  - T-Lymphocytes/immunology
MH  - Vaccination
EDAT- 2000/09/16 11:00
MHDA- 2001/02/28 10:01
CRDT- 2000/09/16 11:00
PHST- 2000/09/16 11:00 [pubmed]
PHST- 2001/02/28 10:01 [medline]
PHST- 2000/09/16 11:00 [entrez]
AID - S0753-3322(00)80050-5 [pii]
AID - 10.1016/S0753-3322(00)80050-5 [doi]
PST - ppublish
SO  - Biomed Pharmacother. 2000 Jul;54(6):291-8. doi: 10.1016/S0753-3322(00)80050-5.