PMID- 10992259
OWN - NLM
STAT- MEDLINE
DCOM- 20001109
LR  - 20131121
IS  - 0040-3709 (Print)
IS  - 0040-3709 (Linking)
VI  - 62
IP  - 4
DP  - 2000 Oct
TI  - Pathogenesis of retinoic acid-induced abnormal pad patterns on mouse volar skin.
PG  - 181-8
AB  - BACKGROUND: Abnormal dermatoglyphs on human volar skin have been reported in many 
      syndromes, but little is known about the pathogenesis. Patterns of pads on rodent 
      limb volar skin are homologous to human dermatoglyphs. METHODS: In previous 
      studies, we showed that transplacental exposure to teratogens induced abnormal 
      pads in mouse fetuses. Moreover, teratogens caused abnormal pad patterns at 
      levels below those that caused skeletal malformations. In this study, we examined 
      morphology and cytokinetics in developing abnormal pads. Pregnant mice were 
      treated with all-trans-retinoic acid at 20 mg/kg orally at embryonal day (E) 12.5 
      (vaginal plug = E0). The hindlimbs of the embryos were harvested and observed 
      under a light microscope and by scanning electron microscopy. Cell proliferation 
      and cell death were estimated by 5-bromo-2'-deoxyuridine (BrdU) labeling, Nile 
      blue A vital staining, and terminal deoxynucleotidyl transferase-mediated dUTP 
      nick end labeling (TUNEL). RESULTS: Retinoic acid induced aplasia of the fibular 
      tarsal pad and supernumerary interdigital pads on hindlimbs. Cell proliferation 
      was observed in the area of developing pad, but cell death was very rarely seen 
      in either normal or abnormal pads. CONCLUSIONS: Retinoic acid disturbed pad 
      patterning as a whole rather than individual pad formation.
CI  - Copyright 2000 Wiley-Liss, Inc.
FAU - Mori, N
AU  - Mori N
AD  - Department of Orthopaedic Surgery, Hiroshima University School of Medicine, 
      Hiroshima, Japan. mori@mcai.med.hiroshima-u.ac.jp
FAU - Tsugane, M H
AU  - Tsugane MH
FAU - Yamashita, K
AU  - Yamashita K
FAU - Ikuta, Y
AU  - Ikuta Y
FAU - Yasuda, M
AU  - Yasuda M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Teratology
JT  - Teratology
JID - 0153257
RN  - 5688UTC01R (Tretinoin)
RN  - G34N38R2N1 (Bromodeoxyuridine)
SB  - IM
CIN - Teratology. 2000 Oct;62(4):178-80. PMID: 10992258
MH  - Abnormalities, Drug-Induced/*etiology/pathology
MH  - Animals
MH  - Bromodeoxyuridine/metabolism
MH  - Cell Death/drug effects
MH  - Cell Survival/drug effects
MH  - Embryo, Mammalian/*drug effects
MH  - Female
MH  - Fibula/pathology/ultrastructure
MH  - Foot Deformities, Congenital/*chemically induced/pathology
MH  - Hindlimb/*abnormalities/drug effects/embryology
MH  - In Situ Nick-End Labeling/methods
MH  - Mice
MH  - Microscopy, Electron, Scanning
MH  - Pregnancy
MH  - Skin/*drug effects/embryology
MH  - Skin Abnormalities/*chemically induced/pathology
MH  - Tretinoin/*toxicity
EDAT- 2000/09/19 11:00
MHDA- 2001/02/28 10:01
CRDT- 2000/09/19 11:00
PHST- 2000/09/19 11:00 [pubmed]
PHST- 2001/02/28 10:01 [medline]
PHST- 2000/09/19 11:00 [entrez]
AID - 10.1002/1096-9926(200010)62:4<181::AID-TERA3>3.0.CO;2-I [pii]
AID - 10.1002/1096-9926(200010)62:4<181::AID-TERA3>3.0.CO;2-I [doi]
PST - ppublish
SO  - Teratology. 2000 Oct;62(4):181-8. doi: 
      10.1002/1096-9926(200010)62:4<181::AID-TERA3>3.0.CO;2-I.