PMID- 10992302
OWN - NLM
STAT- MEDLINE
DCOM- 20001107
LR  - 20191025
IS  - 1045-2257 (Print)
IS  - 1045-2257 (Linking)
VI  - 29
IP  - 3
DP  - 2000 Nov
TI  - Combined immunophenotyping and FISH identifies the involvement of B-cells in 5q- 
      syndrome.
PG  - 276-80
AB  - The 5q- syndrome is a distinct subtype of myelodysplastic syndrome (MDS) 
      characterized by refractory anemia, deletion of the long arm of chromosome 5, 
      del(5q), as the sole cytogenetic abnormality, and a low frequency of 
      transformation to acute leukemia. Using combined immunophenotyping and 
      fluorescence in situ hybridization (FISH), studies were carried out on bone 
      marrow smears of three 5q- syndrome cases to identify the cell lineages carrying 
      the 5q deletion. In all three cases, the granulocytic, monocytic, and erythroid 
      lineages possessed the del(5q) clonal marker, whereas the T-lymphocytes did not. 
      Interestingly, in one case, cells of B-lymphoid lineage also showed the presence 
      of the del(5q). This is the first report to date showing involvement of an 
      acquired 5q deletion associated with MDS in B-cells. This result suggests that in 
      some cases, MDS arises in a multipotent cell with a capacity to differentiate 
      into both myeloid and lymphoid cells.
CI  - Copyright 2000 Wiley-Liss, Inc.
FAU - Jaju, R J
AU  - Jaju RJ
AD  - Leukaemia Research Fund Molecular Haematology Unit, Clinical Laboratory Sciences, 
      University of Oxford, John Radcliffe Hospital, Oxford, UK. 
      rina.jaju@cellular-science.ox.ac.uk
FAU - Jones, M
AU  - Jones M
FAU - Boultwood, J
AU  - Boultwood J
FAU - Kelly, S
AU  - Kelly S
FAU - Mason, D Y
AU  - Mason DY
FAU - Wainscoat, J S
AU  - Wainscoat JS
FAU - Kearney, L
AU  - Kearney L
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Genes Chromosomes Cancer
JT  - Genes, chromosomes & cancer
JID - 9007329
RN  - 0 (Biomarkers)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Anemia, Refractory/diagnosis/genetics/*pathology
MH  - B-Lymphocytes/*pathology
MH  - Biomarkers
MH  - Bone Marrow Cells/metabolism/pathology
MH  - *Chromosome Deletion
MH  - Chromosomes, Human, Pair 5/*genetics
MH  - Female
MH  - Humans
MH  - *Immunophenotyping/methods
MH  - *In Situ Hybridization, Fluorescence/methods
MH  - Middle Aged
MH  - Myelodysplastic Syndromes/diagnosis/*genetics/*pathology
EDAT- 2000/09/19 11:00
MHDA- 2001/02/28 10:01
CRDT- 2000/09/19 11:00
PHST- 2000/09/19 11:00 [pubmed]
PHST- 2001/02/28 10:01 [medline]
PHST- 2000/09/19 11:00 [entrez]
AID - 10.1002/1098-2264(2000)9999:9999<::AID-GCC1035>3.0.CO;2-L [pii]
AID - 10.1002/1098-2264(2000)9999:9999<::aid-gcc1035>3.0.co;2-l [doi]
PST - ppublish
SO  - Genes Chromosomes Cancer. 2000 Nov;29(3):276-80. doi: 
      10.1002/1098-2264(2000)9999:9999<::aid-gcc1035>3.0.co;2-l.