PMID- 10992417
OWN - NLM
STAT- MEDLINE
DCOM- 20001005
LR  - 20141120
IS  - 0022-5347 (Print)
IS  - 0022-5347 (Linking)
VI  - 164
IP  - 4
DP  - 2000 Oct
TI  - Tachykininergic excitatory neurotransmission in the pig intravesical ureter.
PG  - 1371-5
AB  - PURPOSE: The present investigation was designed to study the role played by 
      neurokinin A (NKA) in the non adrenergic non cholinergic (NANC) neurotransmission 
      of the pig intravesical ureter. MATERIALS AND METHODS: We used 
      immunohistochemical techniques to evidence the distribution of NKA-immunoreactive 
      (NKA-IR) fibers in the pig intravesical ureter. We have also performed isolated 
      organ bath experiments to release endogenous tachykinins from ureteral nerves and 
      to characterize the functionally active receptor through which endogenous ligands 
      evoke contraction, and to show the effect of exogenous tachykinins on 
      intravesical ureteral smooth muscle. RESULTS: NKA-IR fibers were found 
      penetrating through ureteral adventitia and distributed in the subepithelial and 
      muscular layers. NKA-IR fibers were not found around small arteries supplying the 
      ureter or in the associated intramural ganglia. Electrical field stimulation 
      (EFS, 1 ms duration, 2 to 16 Hz, 20 s trains) performed in NANC conditions evoked 
      frequency-dependent contractions which were reduced by capsaicin (10-5 M) and GR 
      94800 (3 x 10-8 M), sensory neurotoxin and NK2 receptor antagonist, respectively. 
      Contractions to EFS were abolished by tetrodotoxin (10-6 M). Exogenous NKA and 
      substance P (SP) induced dose-dependent contractions, characterized by an 
      increase of the ureteral basal tone, NKA being more potent than SP. CONCLUSIONS: 
      These results suggest that tachykinins, especially NKA, released from 
      capsaicin-sensitive primary afferents, are involved in the NANC excitatory 
      neurotransmission, contracting the smooth muscle via NK2 receptors activation, in 
      the pig intravesical ureter. NKA at this level does not seem to participate in 
      the regulation of local blood flow, plasmatic extravasation or ganglionar 
      transmission.
FAU - Bustamante, S
AU  - Bustamante S
AD  - Servicio de Urologia, Hospital Universitario de Getafe, Departamentos de 
      Fisiologia, Toxicologia y Farmacologia and Anatomia y Anatomia Patologica 
      Comparada, Facultad de Veterinaria, Universidad Complutense de Madrid, Spain.
FAU - Orensanz, L M
AU  - Orensanz LM
FAU - Barahona, M V
AU  - Barahona MV
FAU - Contreras, J
AU  - Contreras J
FAU - Garcia-Sacristan, A
AU  - Garcia-Sacristan A
FAU - Hernandez, M
AU  - Hernandez M
LA  - eng
PT  - Journal Article
PL  - United States
TA  - J Urol
JT  - The Journal of urology
JID - 0376374
RN  - 33507-63-0 (Substance P)
RN  - 86933-74-6 (Neurokinin A)
SB  - IM
MH  - Animals
MH  - Female
MH  - Immunohistochemistry
MH  - In Vitro Techniques
MH  - Male
MH  - Neurokinin A/*physiology
MH  - Substance P/physiology
MH  - Swine
MH  - Ureter/*innervation
EDAT- 2000/09/19 11:00
MHDA- 2000/10/07 11:01
CRDT- 2000/09/19 11:00
PHST- 2000/09/19 11:00 [pubmed]
PHST- 2000/10/07 11:01 [medline]
PHST- 2000/09/19 11:00 [entrez]
AID - S0022-5347(05)67201-1 [pii]
PST - ppublish
SO  - J Urol. 2000 Oct;164(4):1371-5.