PMID- 10993692
OWN - NLM
STAT- MEDLINE
DCOM- 20001026
LR  - 20091119
IS  - 0014-4886 (Print)
IS  - 0014-4886 (Linking)
VI  - 165
IP  - 2
DP  - 2000 Oct
TI  - Changes in truncated trkB and p75 receptor expression in the rat spinal cord 
      following spinal cord hemisection and spinal cord hemisection plus neurotrophin 
      treatment.
PG  - 327-41
AB  - Although numerous studies have examined the effects of neurotrophin treatment 
      following spinal cord injury, few have examined the changes that occur in the 
      neurotrophin receptors following either such damage or neurotrophin treatment. To 
      determine what changes occur in neurotrophin receptor expression following spinal 
      cord damage, adult rats received a midthoracic spinal cord hemisection alone or 
      in combination with intrathecal application of brain-derived neurotrophic factor 
      (BDNF) or neurotrophin-3 (NT-3). Using immunohistochemical and in situ 
      hybridization techniques, p75, trkA, trkB, and trkC receptor expression was 
      examined throughout the spinal cord. Results showed that trkA, full-length trkB, 
      and trkC receptors were not present in the lesion site but had a normal 
      expression pattern in uninjured parts of the spinal cord. In contrast, p75 
      receptor expression occurred on Schwann cells throughout the lesion site. BDNF 
      and NT-3 (but not saline) applied to the lesion site increased this expression. 
      In addition, the truncated trkB receptor was expressed in the border between the 
      lesion and intact spinal cord. Truncated trkB receptor expression was also 
      increased throughout the white matter ipsilateral to the lesion and BDNF (but not 
      NT-3 or saline) prevented this increase. The study is the first to show changes 
      in truncated trkB receptor expression that extend beyond the site of a spinal 
      cord lesion and is one of the first to show that BDNF and NT-3 affect Schwann 
      cells and/or p75 expression following spinal cord damage. These results indicate 
      that changes in neurotrophin receptor expression following spinal cord injury 
      could influence the availability of neurotrophins at the lesion site. In 
      addition, neurotrophins may affect their own availability to damaged neurons by 
      altering the expression of the p75 and truncated trkB receptor.
CI  - Copyright 2000 Academic Press.
FAU - King, V R
AU  - King VR
AD  - Neuroscience Section, Division of Biomedical Sciences, Queen Mary and Westfield 
      College, Mile End Road, London, E1 4NS, United Kingdom.
FAU - Bradbury, E J
AU  - Bradbury EJ
FAU - McMahon, S B
AU  - McMahon SB
FAU - Priestley, J V
AU  - Priestley JV
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Exp Neurol
JT  - Experimental neurology
JID - 0370712
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Neurotrophin 3)
RN  - 0 (RNA, Messenger)
RN  - 0 (Receptor, Nerve Growth Factor)
RN  - EC 2.7.10.1 (Receptor, trkB)
SB  - IM
MH  - Animals
MH  - Brain-Derived Neurotrophic Factor/pharmacology/therapeutic use
MH  - Male
MH  - Neurotrophin 3/pharmacology/therapeutic use
MH  - RNA, Messenger/drug effects/metabolism
MH  - Rats
MH  - Rats, Wistar
MH  - Receptor, Nerve Growth Factor/drug effects/*metabolism
MH  - Receptor, trkB/drug effects/*metabolism
MH  - Spinal Cord/drug effects/*metabolism
MH  - Spinal Cord Injuries/drug therapy/*metabolism
EDAT- 2000/09/20 11:00
MHDA- 2001/02/28 10:01
CRDT- 2000/09/20 11:00
PHST- 2000/09/20 11:00 [pubmed]
PHST- 2001/02/28 10:01 [medline]
PHST- 2000/09/20 11:00 [entrez]
AID - S0014-4886(00)97480-6 [pii]
AID - 10.1006/exnr.2000.7480 [doi]
PST - ppublish
SO  - Exp Neurol. 2000 Oct;165(2):327-41. doi: 10.1006/exnr.2000.7480.