PMID- 10993939
OWN - NLM
STAT- MEDLINE
DCOM- 20001115
LR  - 20200213
IS  - 0022-1317 (Print)
IS  - 0022-1317 (Linking)
VI  - 81
IP  - Pt 10
DP  - 2000 Oct
TI  - Variations in the major envelope glycoprotein GP5 of Czech strains of porcine 
      reproductive and respiratory syndrome virus.
PG  - 2497-2502
LID - 10.1099/0022-1317-81-10-2497 [doi]
AB  - The major envelope glycoprotein genes (ORF5) of seven Czech isolates of porcine 
      reproductive and respiratory syndrome virus (PRRSV) were amplified and their 
      nucleotide sequences were determined. ORF5 displayed nucleotide and amino acid 
      identities of 87.5-100% and 87. 6-100%, respectively, among the isolates. In a 
      phylogenetic tree, all European isolates were grouped in a genotype distinct from 
      that of reference American strains (VR-2332, IAF-Klop). Among the European 
      isolates, two different clades were identified. Two Czech isolates (V-501 and 
      V-503) and Italian strain PRRSV 2156 fell into one clade. The remaining European 
      strains comprised the second clade. Surprisingly, two separately clustered 
      strains (V-501 and V-516) were isolated from the same herd. Additionally, the 
      possible effect of in vitro cultivation on the nucleotide sequence was analysed. 
      Nine point mutations in the ORF5 region resulted from 152 in vitro passages of 
      the V-502 isolate in MARC-145 cells.
FAU - Indik, Stanislav
AU  - Indik S
AD  - Veterinary Research Institute, Hudcova 70, Brno 621 32, Czech Republic1.
FAU - Valicek, Lubomir
AU  - Valicek L
AD  - Veterinary Research Institute, Hudcova 70, Brno 621 32, Czech Republic1.
FAU - Klein, Dieter
AU  - Klein D
AD  - University of Veterinary Sciences, Veterinarplatz 1, A-1210 Vienna, Austria2.
FAU - Klanova, Jana
AU  - Klanova J
AD  - Faculty of Science, Masaryk University, Kotlarska 2, Brno 611 37, Czech 
      Republic3.
LA  - eng
SI  - GENBANK/AF253531
SI  - GENBANK/AF253532
SI  - GENBANK/AF253533
SI  - GENBANK/AF253534
SI  - GENBANK/AF253535
SI  - GENBANK/AF253536
SI  - GENBANK/AF253537
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - J Gen Virol
JT  - The Journal of general virology
JID - 0077340
RN  - 0 (DNA, Viral)
RN  - 0 (Membrane Glycoproteins)
RN  - 0 (Viral Envelope Proteins)
RN  - 0 (glycoprotein 5, PRRSV)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Base Sequence
MH  - Czech Republic
MH  - DNA, Viral/chemistry
MH  - Membrane Glycoproteins/*genetics
MH  - Molecular Sequence Data
MH  - Point Mutation
MH  - Porcine respiratory and reproductive syndrome virus/*genetics
MH  - Sequence Alignment
MH  - Swine
MH  - Viral Envelope Proteins/*genetics
EDAT- 2000/09/20 11:00
MHDA- 2001/02/28 10:01
CRDT- 2000/09/20 11:00
PHST- 2000/09/20 11:00 [pubmed]
PHST- 2001/02/28 10:01 [medline]
PHST- 2000/09/20 11:00 [entrez]
AID - 10.1099/0022-1317-81-10-2497 [doi]
PST - ppublish
SO  - J Gen Virol. 2000 Oct;81(Pt 10):2497-2502. doi: 10.1099/0022-1317-81-10-2497.