PMID- 10994713 OWN - NLM STAT- MEDLINE DCOM- 20010118 LR - 20190513 IS - 0022-1503 (Print) IS - 0022-1503 (Linking) VI - 91 IP - 5 DP - 2000 Sep-Oct TI - Characterization and chromosomal distribution of a novel satellite DNA sequence of Japanese quail (Coturnix coturnix japonica). PG - 412-5 AB - A novel satellite DNA sequence of Japanese quail (Coturnix coturnix japonica) was isolated from genomic DNA digested with restriction endonuclease, Bg/II. Sequence analysis of three different-size clones revealed the presence of a tandem array of a GC-rich 41 bp repeated element. This sequence was localized by fluorescence in situ hybridization (FISH) primarily to microchromosomes of Japanese quail (2n = 78); approximately 50 of the 66 microchromosomes showed positive signals, although hybridization signals were also detected on chromosomes 4 and W. This satellite DNA did not cross-hybridize with genomic DNA of chicken (Gallus gallus) and Chinese painted quail (Excalfactoria chinensis) under moderately stringent conditions, suggesting that this class of repetitive DNA sequences was species specific and fairly divergent in Galliformes species. FAU - Tanaka, K AU - Tanaka K AD - Laboratory of Animal Genetics, Graduate School of Bioagricultural Sciences, Nagoya University, Chikusa, Japan. kztana@agr.nagoya-u.ac.jp FAU - Suzuki, T AU - Suzuki T FAU - Nojiri, T AU - Nojiri T FAU - Yamagata, T AU - Yamagata T FAU - Namikawa, T AU - Namikawa T FAU - Matsuda, Y AU - Matsuda Y LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't PL - United States TA - J Hered JT - The Journal of heredity JID - 0375373 RN - 0 (DNA, Satellite) SB - IM MH - Animals MH - Base Sequence MH - Blotting, Southern MH - Cells, Cultured MH - Chromosome Mapping/*methods MH - Chromosomes/*genetics MH - Cloning, Molecular MH - Coturnix/*genetics MH - DNA, Satellite/*genetics MH - Female MH - In Situ Hybridization, Fluorescence MH - Molecular Sequence Data MH - Sequence Homology, Nucleic Acid EDAT- 2000/09/20 11:00 MHDA- 2001/02/28 10:01 CRDT- 2000/09/20 11:00 PHST- 2000/09/20 11:00 [pubmed] PHST- 2001/02/28 10:01 [medline] PHST- 2000/09/20 11:00 [entrez] AID - 10.1093/jhered/91.5.412 [doi] PST - ppublish SO - J Hered. 2000 Sep-Oct;91(5):412-5. doi: 10.1093/jhered/91.5.412.