PMID- 10995793
OWN - NLM
STAT- MEDLINE
DCOM- 20001012
LR  - 20181113
IS  - 0021-9738 (Print)
IS  - 1558-8238 (Electronic)
IS  - 0021-9738 (Linking)
VI  - 106
IP  - 6
DP  - 2000 Sep
TI  - Alveolar epithelial cell chemokine expression triggered by antigen-specific 
      cytolytic CD8(+) T cell recognition.
PG  - R49-58
AB  - CD8(+) T lymphocyte responses are a critical arm of the immune response to 
      respiratory virus infection and may play a role in the pathogenesis of 
      interstitial lung disease. We have shown that CD8(+) T cells induce significant 
      lung injury in the absence of virus infection by adoptive transfer into mice with 
      alveolar expression of a viral transgene. The injury is characterized by the 
      parenchymal infiltration of host cells, primarily macrophages, which correlates 
      with physiologic deficits in transgenic animals. CD8(+) T cell-mediated lung 
      injury can occur in the absence of perforin and Fas expression as long as 
      TNF-alpha is available. Here, we show that the effect of TNF-alpha expressed by 
      CD8(+) T cells is mediated not exclusively by cytotoxicity, but also through the 
      activation of alveolar target cells and their expression of inflammatory 
      mediators. CD8(+) T cell recognition of alveolar cells in vitro triggered 
      monocyte chemoattractant protein-1 (MCP-1) and macrophage inflammatory protein-2 
      (MIP-2) expression in the targets, which was mediated by TNF-alpha. 
      Antigen-dependent alveolar MCP-1 expression was observed in vivo as early as 3 
      hours after CD8(+) T cell transfer and depended upon TNF-R1 expression in 
      transgenic recipients. MCP-1 neutralization significantly reduced parenchymal 
      infiltration after T cell transfer. We conclude that alveolar epithelial cells 
      actively participate in the inflammation and lung injury associated with CD8(+) T 
      cell recognition of alveolar antigens.
FAU - Zhao, M Q
AU  - Zhao MQ
AD  - Department of Medicine, The Beirne B. Carter Center for Immunology Research, 
      University of Virginia School of Medicine, Charlottesville, Virginia 22908, USA.
FAU - Stoler, M H
AU  - Stoler MH
FAU - Liu, A N
AU  - Liu AN
FAU - Wei, B
AU  - Wei B
FAU - Soguero, C
AU  - Soguero C
FAU - Hahn, Y S
AU  - Hahn YS
FAU - Enelow, R I
AU  - Enelow RI
LA  - eng
GR  - R01 HL058660/HL/NHLBI NIH HHS/United States
GR  - R29 HL058660/HL/NHLBI NIH HHS/United States
GR  - HL58660/HL/NHLBI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Clin Invest
JT  - The Journal of clinical investigation
JID - 7802877
RN  - 0 (Antigens)
RN  - 0 (Chemokine CCL2)
RN  - 0 (Chemokine CXCL2)
RN  - 0 (Chemokines)
RN  - 0 (Cxcl2 protein, mouse)
RN  - 0 (RNA, Messenger)
RN  - 0 (Receptors, Antigen, T-Cell)
RN  - 0 (Receptors, Tumor Necrosis Factor)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - EC 3.4.24.- (Metalloendopeptidases)
SB  - IM
CIN - J Clin Invest. 2000 Sep;106(6):741-3. PMID: 10995783
MH  - Animals
MH  - Antigens/*immunology/metabolism
MH  - CD8-Positive T-Lymphocytes/*immunology/metabolism
MH  - Cell Line
MH  - Chemokine CCL2/genetics/immunology/metabolism
MH  - Chemokine CXCL2
MH  - Chemokines/genetics/immunology/*metabolism
MH  - Epithelial Cells/immunology/*metabolism/pathology
MH  - Gene Expression Regulation/drug effects
MH  - Histocytochemistry
MH  - In Situ Hybridization
MH  - Inflammation/immunology
MH  - Lymphocyte Activation
MH  - Metalloendopeptidases/antagonists & inhibitors
MH  - Mice
MH  - Mice, Transgenic
MH  - Pulmonary Alveoli/*immunology/*metabolism/pathology
MH  - RNA, Messenger/analysis
MH  - Receptors, Antigen, T-Cell/immunology
MH  - Receptors, Tumor Necrosis Factor/metabolism
MH  - Tumor Necrosis Factor-alpha/metabolism/pharmacology
PMC - PMC381394
EDAT- 2000/09/21 11:00
MHDA- 2000/10/14 11:01
PMCR- 2000/09/15
CRDT- 2000/09/21 11:00
PHST- 2000/09/21 11:00 [pubmed]
PHST- 2000/10/14 11:01 [medline]
PHST- 2000/09/21 11:00 [entrez]
PHST- 2000/09/15 00:00 [pmc-release]
AID - 09786 [pii]
AID - 10.1172/JCI9786 [doi]
PST - ppublish
SO  - J Clin Invest. 2000 Sep;106(6):R49-58. doi: 10.1172/JCI9786.