PMID- 10995881
OWN - NLM
STAT- MEDLINE
DCOM- 20001113
LR  - 20190605
IS  - 1019-6439 (Print)
IS  - 1019-6439 (Linking)
VI  - 17
IP  - 4
DP  - 2000 Oct
TI  - High frequency of tetraploidy detected in malignant melanoma of Japanese patients 
      by fluorescence in situ hybridization.
PG  - 707-15
AB  - Aneuploidy and hyperploidy are often detected in malignant melanoma by 
      cytogenetic analysis and flow cytometric analysis of DNA content. To determine 
      the ploidy of cells in surgical specimens of melanin-producing tumors of Japanese 
      patients, we performed fluorescence in situ hybridization (FISH) using touch 
      smear technique to count the number of chromosomes 18 and X + Y in interphase 
      nuclei using alpha-satellite DNA probes, D18Z1, DXZ1 and DYZ3. A normal 
      melanocyte strain showed two D18Z1 and two [DXZ1+DYZ3] signals per nucleus, 
      indicating 2N, and a malignant melanoma cell line showed 4 per nucleus, 
      indicating 4N, consistent with results of cytogenetic and flow cytometric 
      analyses. Therefore we employed this FISH method to analyze ploidy of surgical 
      specimens. Specimens obtained from 8 patients with nevus cell nevus showed 2 FISH 
      signals per nucleus. On the other hand, in all specimens obtained from 8 patients 
      with malignant melanoma (6 primary and 2 metastatic melanoma), 65-90% of cells 
      exhibited 4 signals per nucleus, indicating 4N. Histopathologically, 50-70% of 
      cells were identified as malignant melanoma cells, indicating that our FISH 
      method is effective to detect melanoma cells in tissue. We also analyzed allelic 
      loss of the p53 gene by FISH with a p53 locus-specific probe and mutation of the 
      p53 gene by immunostaining since mutation and deletion of the p53 gene may cause 
      hyperploidy. All specimens except one obtained from a case with young-onset 
      metastatic melanoma exhibited no allelic losses or negative p53 staining, showing 
      the p53 gene was intact. These results indicate that tetraploidy, not caused by 
      p53 mutation or deletion, is commonly found in malignant melanoma of Japanese 
      patients. It is also suggested that there is no positive relationship between 
      tetraploidy and poorer prognosis, and mutation and allelic loss of the p53 gene 
      might be markers of aggressive form of malignant melanoma.
FAU - Satoh, S
AU  - Satoh S
AD  - Department of Dermatology, Hyogo College of Medicine, Nishinomiya 663-8501, 
      Japan.
FAU - Hashimoto-Tamaoki, T
AU  - Hashimoto-Tamaoki T
FAU - Furuyama, J
AU  - Furuyama J
FAU - Mihara, K
AU  - Mihara K
FAU - Namba, M
AU  - Namba M
FAU - Kitano, Y
AU  - Kitano Y
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Greece
TA  - Int J Oncol
JT  - International journal of oncology
JID - 9306042
RN  - 0 (Tumor Suppressor Protein p53)
RN  - 9007-49-2 (DNA)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - DNA/genetics/metabolism
MH  - Female
MH  - Flow Cytometry
MH  - Humans
MH  - Immunohistochemistry
MH  - In Situ Hybridization, Fluorescence
MH  - Leukocytes/cytology/metabolism
MH  - Loss of Heterozygosity
MH  - Male
MH  - Melanoma/genetics/metabolism/*pathology
MH  - Middle Aged
MH  - *Polyploidy
MH  - Skin Neoplasms/genetics/metabolism/*pathology
MH  - Tumor Cells, Cultured
MH  - Tumor Suppressor Protein p53/analysis/genetics
EDAT- 2000/09/21 11:00
MHDA- 2001/02/28 10:01
CRDT- 2000/09/21 11:00
PHST- 2000/09/21 11:00 [pubmed]
PHST- 2001/02/28 10:01 [medline]
PHST- 2000/09/21 11:00 [entrez]
AID - 10.3892/ijo.17.4.707 [doi]
PST - ppublish
SO  - Int J Oncol. 2000 Oct;17(4):707-15. doi: 10.3892/ijo.17.4.707.