PMID- 10996021
OWN - NLM
STAT- MEDLINE
DCOM- 20010118
LR  - 20190921
IS  - 0162-3109 (Print)
IS  - 0162-3109 (Linking)
VI  - 49
IP  - 3
DP  - 2000 Sep
TI  - Antisense IRAK-1 oligonucleotide blocks activation of NF-kappa B and AP-1 induced 
      by IL-18.
PG  - 241-6
AB  - Interleukin-18 (IL-18) is a novel proinflammatory cytokine. Most of the immune 
      and inflammatory genes induced by IL-18 are nuclear factor-kappaB (NF-kappaB)- 
      and activator protein-1 (AP-1)-regulated. Interleukin-1 receptor-associated 
      kinase-1 (IRAK-1) has recently been shown to be involved in IL-18-stimulated 
      activation of NF-kappaB and AP-1. The purpose of this study is to investigate the 
      effects of preventing IRAK-1 expression by antisense IRAK-1 oligodeoxynucleotide 
      (ODN) on IL-18-stimulated activation of NF-kappaB and AP-1. Semiquantitative 
      reverse transcription-PCR (RT-PCR) and western blot analysis revealed that 
      antisense IRAK-1 ODN inhibited IRAK-1 mRNA and protein expression (P<0.01). As a 
      result, antisense IRAK-1 ODN attenuated IL-18-induced activation of NF-kappaB and 
      AP-1 as measured by sandwich ELISA in a concentration (1-8 microg ml(-1))- and 
      time (5-24 h)-dependent fashion. These data suggest that antisense IRAK-1 ODN may 
      share a role in the design of antiinflammatory therapeutics.
FAU - Guo, F
AU  - Guo F
AD  - Institute of Pharmaceutic Sciences, The First Military Medical University, 
      510515, Guang Zhou, People's Republic of China.
FAU - Wu, S
AU  - Wu S
LA  - eng
PT  - Journal Article
PL  - Netherlands
TA  - Immunopharmacology
JT  - Immunopharmacology
JID - 7902474
RN  - 0 (Enzyme Inhibitors)
RN  - 0 (Interleukin-18)
RN  - 0 (NF-kappa B)
RN  - 0 (Oligonucleotides, Antisense)
RN  - 0 (Protein Kinase Inhibitors)
RN  - 0 (Receptors, Interleukin-1)
RN  - 0 (Transcription Factor AP-1)
RN  - EC 2.7.- (Protein Kinases)
RN  - EC 2.7.11.1 (Interleukin-1 Receptor-Associated Kinases)
SB  - IM
MH  - Enzyme Activation/drug effects/genetics
MH  - Enzyme Inhibitors/pharmacology
MH  - Gene Expression Regulation/drug effects
MH  - Humans
MH  - Interleukin-1 Receptor-Associated Kinases
MH  - Interleukin-18/*antagonists & inhibitors/*physiology
MH  - NF-kappa B/*antagonists & inhibitors/genetics
MH  - Oligonucleotides, Antisense/*pharmacology
MH  - Protein Kinase Inhibitors
MH  - Protein Kinases/biosynthesis/*genetics/metabolism
MH  - Receptors, Interleukin-1/genetics/*metabolism
MH  - Transcription Factor AP-1/*antagonists & inhibitors/metabolism
MH  - Tumor Cells, Cultured
EDAT- 2000/09/21 11:00
MHDA- 2001/02/28 10:01
CRDT- 2000/09/21 11:00
PHST- 2000/09/21 11:00 [pubmed]
PHST- 2001/02/28 10:01 [medline]
PHST- 2000/09/21 11:00 [entrez]
AID - S0162310900002009 [pii]
AID - 10.1016/s0162-3109(00)00200-9 [doi]
PST - ppublish
SO  - Immunopharmacology. 2000 Sep;49(3):241-6. doi: 10.1016/s0162-3109(00)00200-9.