PMID- 10998432
OWN - NLM
STAT- MEDLINE
DCOM- 20001025
LR  - 20190605
IS  - 1107-3756 (Print)
IS  - 1107-3756 (Linking)
VI  - 6
IP  - 4
DP  - 2000 Oct
TI  - Use of real-time PCR to monitor human herpesvirus 6 reactivation after allogeneic 
      bone marrow transplantation.
PG  - 427-32
AB  - Reactivation of human herpesvirus 6 (HHV-6) is common following allogeneic marrow 
      transplantation, however, little is known about the immune control and pathogenic 
      potential of HHV-6 infection after transplantation. In order to determine whether 
      reactivation of HHV-6 plays an important role in the development of complications 
      in patients undergoing allogeneic bone marrow transplantation or not, we 
      developed a very rapid quantification of viral DNA using a LightCycler. The 
      amount of viral DNA was determined using a supernatant of a chronically infected 
      cell line [TaY(OK)] which contains a known amount of viral DNA. Peripheral blood 
      cells were collected from 5 patients undergoing allogeneic bone marrow 
      transplantation once before transplant and once per week after transplant for 
      8-24 weeks. The real-time PCR system revealed that there was a linear correlation 
      in the range of 101 to 105 molecules of reference. Using this system, we have 
      found the presence of non-diagnosed HHV-6 reactivation as well as symptomatic 
      infection, indicating the potential for routine implementation of this technology 
      for laboratory diagnosis of HHV-6 infection. Our study shows that this method of 
      rapid quantification of HHV-6 genomes by the real-time PCR using a LightCycler 
      may be useful not only to understand the reconstitution of the immune system 
      following marrow transplantation but also to manage the care of patients.
FAU - Ohyashiki, J H
AU  - Ohyashiki JH
AD  - Department of Virology, Medical Research Institute, Tokyo Medical and Dental 
      University, Bunkyo-ku, Tokyo 113-8510, Japan. junko@hh.iij4u.or.jp
FAU - Suzuki, A
AU  - Suzuki A
FAU - Aritaki, K
AU  - Aritaki K
FAU - Nagate, A
AU  - Nagate A
FAU - Shoji, N
AU  - Shoji N
FAU - Ohyashiki, K
AU  - Ohyashiki K
FAU - Ojima, T
AU  - Ojima T
FAU - Abe, K
AU  - Abe K
FAU - Yamamoto, K
AU  - Yamamoto K
LA  - eng
PT  - Journal Article
PL  - Greece
TA  - Int J Mol Med
JT  - International journal of molecular medicine
JID - 9810955
RN  - 0 (DNA, Viral)
RN  - 9007-49-2 (DNA)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Aged
MH  - Base Sequence
MH  - *Bone Marrow Transplantation
MH  - DNA/blood/genetics
MH  - DNA, Viral/blood/genetics
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Herpesviridae Infections/*virology
MH  - Herpesvirus 6, Human/*genetics/growth & development
MH  - Humans
MH  - Male
MH  - Middle Aged
MH  - Polymerase Chain Reaction/*methods
MH  - Reproducibility of Results
MH  - Transplantation, Homologous
MH  - *Virus Activation
EDAT- 2000/09/22 11:00
MHDA- 2001/02/28 10:01
CRDT- 2000/09/22 11:00
PHST- 2000/09/22 11:00 [pubmed]
PHST- 2001/02/28 10:01 [medline]
PHST- 2000/09/22 11:00 [entrez]
AID - 10.3892/ijmm.6.4.427 [doi]
PST - ppublish
SO  - Int J Mol Med. 2000 Oct;6(4):427-32. doi: 10.3892/ijmm.6.4.427.