PMID- 10999533
OWN - NLM
STAT- MEDLINE
DCOM- 20001017
LR  - 20220129
IS  - 0013-9580 (Print)
IS  - 0013-9580 (Linking)
VI  - 41 Suppl 6
DP  - 2000
TI  - Different patterns of induction of fibroblast growth factor-2 and brain-derived 
      neurotrophic factor messenger RNAs during kindling epileptogenesis, and 
      development of a herpes simplex vector for fibroblast growth factor-2 gene 
      transfer in vivo.
PG  - S122-6
AB  - PURPOSE: To investigate the gene expression patterns of brain-derived 
      neurotrophic factor (BDNF) and fibroblast growth factor-2 (FGF-2) in the kindling 
      model, and to construct a replication-defective herpes simplex virus vector to 
      induce expression of FGF-2 in vivo. METHODS: RNase protection assay and herpes 
      simplex virus vector (TH FGF-2) deleted in the immediate-early genes ICP4, ICP22, 
      and ICP27, with FGF-2 inserted in tk under the control of the human 
      cytomegalovirus immediate-early promoter. RESULTS: A single kindling stimulation 
      did not modify BDNF gene expression, whereas it increased FGF-2 messenger RNA 
      (mRNA) levels in the hippocampus, the cortex, and the hypothalamus. BDNF and 
      FGF-2 gene expression were not altered in kindled animals left unstimulated for 1 
      week. In contrast, kindled seizures produced a great increase in hippocampal and 
      cortical BDNF mRNA levels, but FGF-2 mRNA was increased only in the ipsilateral 
      cortex. Infection of Vero cells with TH FGF-2 resulted in a long-lasting increase 
      in FGF-2 levels. Protein extracts of infected cells induced neuronal 
      differentiation of PC12 cells, indicating that the newly synthesized FGF-2 was 
      biologically active. Robust transient transgene expression was observed in the 
      rat hippocampus after inoculation with TH FGF-2 in the absence of significant 
      toxicity. CONCLUSIONS: BDNF and FGF-2 are recruited at different stages of 
      kindling and, accordingly, may play different roles in the adaptive changes 
      taking place during epileptogenesis. TH FGF-2 is suitable for studies of FGF-2 
      involvement in kindling epileptogenesis.
FAU - Bregola, G
AU  - Bregola G
AD  - Department of Experimental and Clinical Medicine, Biotechnology Center, 
      University of Ferrara, Italy.
FAU - Frigati, L
AU  - Frigati L
FAU - Zucchini, S
AU  - Zucchini S
FAU - Simonato, M
AU  - Simonato M
LA  - eng
GR  - E.0954/TI_/Telethon/Italy
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Epilepsia
JT  - Epilepsia
JID - 2983306R
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (RNA, Messenger)
RN  - 103107-01-3 (Fibroblast Growth Factor 2)
SB  - IM
MH  - Animals
MH  - Brain-Derived Neurotrophic Factor/*genetics/*metabolism
MH  - Defective Viruses/genetics
MH  - Epilepsy/chemically induced/*genetics/*metabolism
MH  - Fibroblast Growth Factor 2/*genetics/*metabolism
MH  - Gene Expression
MH  - *Gene Transfer Techniques
MH  - Genetic Vectors/genetics
MH  - Hippocampus/chemistry/metabolism
MH  - In Situ Hybridization
MH  - Kindling, Neurologic/*metabolism
MH  - Male
MH  - RNA, Messenger/analysis/biosynthesis/metabolism
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Simplexvirus/*genetics
MH  - Transgenes/genetics
EDAT- 2000/09/22 11:00
MHDA- 2000/10/21 11:01
CRDT- 2000/09/22 11:00
PHST- 2000/09/22 11:00 [pubmed]
PHST- 2000/10/21 11:01 [medline]
PHST- 2000/09/22 11:00 [entrez]
AID - 10.1111/j.1528-1157.2000.tb01570.x [doi]
PST - ppublish
SO  - Epilepsia. 2000;41 Suppl 6:S122-6. doi: 10.1111/j.1528-1157.2000.tb01570.x.