PMID- 11000220
OWN - NLM
STAT- MEDLINE
DCOM- 20001012
LR  - 20190508
IS  - 0022-538X (Print)
IS  - 1098-5514 (Electronic)
IS  - 0022-538X (Linking)
VI  - 74
IP  - 20
DP  - 2000 Oct
TI  - Rous sarcoma virus DR posttranscriptional elements use a novel RNA export 
      pathway.
PG  - 9507-14
AB  - Rous sarcoma virus (RSV), a simple retrovirus, needs to export unspliced viral 
      RNA from the nucleus to the cytoplasm, circumventing the host cell restriction on 
      cytoplasmic expression of intron-containing RNA. The cytoplasmic accumulation of 
      full-length viral RNA is promoted by two cis-acting direct repeat (DR) elements 
      that flank the src gene; at least one copy of the DR sequence is necessary for 
      viral replication. We show here that the DR mediates export of a reporter 
      construct from the nucleus, suggesting it is a constitutive transport element 
      (CTE). In contrast, human immunodeficiency virus type 1 (HIV-1) and other complex 
      retroviruses encode accessory proteins, Rev or Rex, which promote export of 
      incompletely spliced viral transcripts. This RNA export pathway is CRM1 dependent 
      and can be blocked by the cytotoxic agent leptomycin B. We show here that 
      DR-mediated export is CRM1 independent, suggesting that RSV uses a different 
      export pathway from that of HIV-1 and other complex retroviruses. The simian 
      retroviruses have a CTE which interacts with the cellular Tap export protein. 
      However, we were unable to detect binding of the RSV DR RNA to Tap, suggesting it 
      may use a different export pathway from that of the simian retroviruses. These 
      data suggest that the RSV DR element uses a novel nucleocytoplasmic export 
      pathway.
FAU - Paca, R E
AU  - Paca RE
AD  - Department of Biology, Johns Hopkins University, Baltimore, Maryland 21218, USA.
FAU - Ogert, R A
AU  - Ogert RA
FAU - Hibbert, C S
AU  - Hibbert CS
FAU - Izaurralde, E
AU  - Izaurralde E
FAU - Beemon, K L
AU  - Beemon KL
LA  - eng
GR  - R01 CA048746/CA/NCI NIH HHS/United States
GR  - 52T32G07231/PHS HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Virol
JT  - Journal of virology
JID - 0113724
RN  - 0 (ATP Binding Cassette Transporter, Subfamily B, Member 2)
RN  - 0 (ATP-Binding Cassette Transporters)
RN  - 0 (RNA, Messenger)
RN  - 0 (RNA, Viral)
RN  - 0 (TAP1 protein, human)
RN  - EC 2.3.1.28 (Chloramphenicol O-Acetyltransferase)
SB  - IM
MH  - ATP Binding Cassette Transporter, Subfamily B, Member 2
MH  - ATP-Binding Cassette Transporters/metabolism
MH  - Animals
MH  - Avian Sarcoma Viruses/*genetics
MH  - Biological Transport
MH  - Cells, Cultured
MH  - Chickens
MH  - Chloramphenicol O-Acetyltransferase/genetics
MH  - HIV-1/genetics
MH  - HeLa Cells
MH  - Humans
MH  - RNA, Messenger/analysis
MH  - RNA, Viral/chemistry/*metabolism
MH  - *Repetitive Sequences, Nucleic Acid
PMC - PMC112380
EDAT- 2000/09/23 11:00
MHDA- 2000/10/14 11:01
PMCR- 2000/10/01
CRDT- 2000/09/23 11:00
PHST- 2000/09/23 11:00 [pubmed]
PHST- 2000/10/14 11:01 [medline]
PHST- 2000/09/23 11:00 [entrez]
PHST- 2000/10/01 00:00 [pmc-release]
AID - 0785 [pii]
AID - 10.1128/jvi.74.20.9507-9514.2000 [doi]
PST - ppublish
SO  - J Virol. 2000 Oct;74(20):9507-14. doi: 10.1128/jvi.74.20.9507-9514.2000.