PMID- 11000533
OWN - NLM
STAT- MEDLINE
DCOM- 20001128
LR  - 20190831
IS  - 0378-1135 (Print)
IS  - 0378-1135 (Linking)
VI  - 76
IP  - 4
DP  - 2000 Oct 20
TI  - Haemophilus somnus-induced IgE in calves vaccinated with commercial monovalent H. 
      somnus bacterins.
PG  - 373-83
AB  - The ability of commercially available Haemophilus somnus bacterins to elicit an 
      immunoglobulin E (IgE) response was examined in healthy calves using 
      enzyme-linked immunosorbent assay (ELISA) and western blotting techniques. Thirty 
      five calves were utilized in this study. Calves in Group 1 (n=7) did not receive 
      any H. somnus vaccination and served as negative controls. Calves in each of 
      Groups 2-5 (n=7 each) were vaccinated on days 0 (primary) and 14 (booster) with 
      one of four commercially available H. somnus bacterins. Sera were harvested on 
      days 0 and 14 and at weekly intervals for a total of 45 days. Sera were tested 
      for the presence of IgE antibodies using a bovine IgE-specific ELISA. Low levels 
      of H. somnus-specific IgE were detected by ELISA in all animals prior to the 
      initiation of the study. All bacterins induced IgE levels that were significantly 
      higher than control levels. Two bacterins elicited higher IgE levels at all time 
      points. Sera were adsorbed against washed whole cells of either Salmonella 
      typhimurium, P. multocida, or H. somnus or extracts of H. somnus. ELISA 
      absorbance values were significantly decreased by adsorption with washed whole 
      cells or extracts of H. somnus, whereas adsorption with other gram-negative 
      bacteria only minimally decreased ELISA absorbance values. These results indicate 
      that commercially available H. somnus bacterins can induce IgE antibody as early 
      as 14 days post-vaccination. This IgE can be detected 45 days after the primary 
      vaccination. Results also indicate that H. somnus-specific IgE antibodies can be 
      found in serum of some cattle, possibly induced by existing or previous 
      sensitization.
FAU - Ruby, K W
AU  - Ruby KW
AD  - United States Department of Agriculture, Animal and Plant Health Inspection 
      Service, Veterinary Services, Center for Veterinary Biologics--Licensing and 
      Policy Development, 510 S. 17th Street, Suite 104, Ames, IA 50010, USA. 
      kevin.w.ruby@usda.gov
FAU - Griffith, R W
AU  - Griffith RW
FAU - Gershwin, L J
AU  - Gershwin LJ
FAU - Kaeberle, M L
AU  - Kaeberle ML
LA  - eng
PT  - Journal Article
PL  - Netherlands
TA  - Vet Microbiol
JT  - Veterinary microbiology
JID - 7705469
RN  - 0 (Antibodies, Bacterial)
RN  - 0 (Antigens, Bacterial)
RN  - 0 (Bacterial Vaccines)
RN  - 37341-29-0 (Immunoglobulin E)
SB  - IM
MH  - Adsorption
MH  - Animals
MH  - Antibodies, Bacterial/biosynthesis
MH  - Antigens, Bacterial/immunology
MH  - Bacterial Vaccines/*immunology
MH  - Cattle/*immunology
MH  - Enzyme-Linked Immunosorbent Assay/veterinary
MH  - Haemophilus/*immunology
MH  - Immunization, Secondary/veterinary
MH  - Immunoglobulin E/*biosynthesis
MH  - Vaccination/*veterinary
EDAT- 2000/09/23 11:00
MHDA- 2001/02/28 10:01
CRDT- 2000/09/23 11:00
PHST- 2000/09/23 11:00 [pubmed]
PHST- 2001/02/28 10:01 [medline]
PHST- 2000/09/23 11:00 [entrez]
AID - S0378-1135(00)00257-1 [pii]
AID - 10.1016/s0378-1135(00)00257-1 [doi]
PST - ppublish
SO  - Vet Microbiol. 2000 Oct 20;76(4):373-83. doi: 10.1016/s0378-1135(00)00257-1.