PMID- 11001311
OWN - NLM
STAT- MEDLINE
DCOM- 20010208
LR  - 20061115
IS  - 0173-0835 (Print)
IS  - 0173-0835 (Linking)
VI  - 21
IP  - 14
DP  - 2000 Aug
TI  - DNA damage promotes mistyping in the allele specific oligonucleotide probing 
      analysis of forensic samples.
PG  - 2969-72
AB  - Five polymerase chain reaction (PCR) products which could not be reliably typed 
      by allele-specific oligonucleotide (ASO) probing at the human leukocyte antigen 
      (HLA) DQA1 locus were analyzed by polyacrylamide gel electrophoresis and direct 
      sequencing. The first method revealed the preferential amplification of only one 
      of the two alleles in two cases. Direct sequencing of PCR products allowed 
      unambiguous genetic typing but a high number of artifacts was observed. Several 
      of these artifacts occurred in the sequences recognized by the ASOs. This finding 
      provides an explanation for the mistyping in the ASO probing procedure because 
      Taq polymerase errors both created new genetic specificities and eliminated 
      site-specific polymorphisms. Reversed-phase HPLC-MS of the five forensic 
      templates showed a high degree of DNA damage. These data together indicate that 
      the risk of mistyping when using the ASO probing procedure cannot be neglected in 
      the forensic analysis of damaged DNA samples.
FAU - Fattorini, P
AU  - Fattorini P
AD  - Institute of Legal Medicine, University of Trieste, Italy. 
      fattorin@univ.trieste.it
FAU - Cossutta, F
AU  - Cossutta F
FAU - Giulianini, P
AU  - Giulianini P
FAU - Edomi, P
AU  - Edomi P
FAU - Previdere, C
AU  - Previdere C
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Germany
TA  - Electrophoresis
JT  - Electrophoresis
JID - 8204476
RN  - 0 (DNA Primers)
RN  - 0 (HLA Antigens)
SB  - IM
MH  - Alleles
MH  - DNA Damage
MH  - DNA Primers
MH  - HLA Antigens/*genetics
MH  - Humans
MH  - Polymerase Chain Reaction/*methods
MH  - Sensitivity and Specificity
EDAT- 2000/09/23 11:00
MHDA- 2001/03/03 10:01
CRDT- 2000/09/23 11:00
PHST- 2000/09/23 11:00 [pubmed]
PHST- 2001/03/03 10:01 [medline]
PHST- 2000/09/23 11:00 [entrez]
AID - 10.1002/1522-2683(20000801)21:14<2969::AID-ELPS2969>3.0.CO;2-7 [pii]
AID - 10.1002/1522-2683(20000801)21:14<2969::AID-ELPS2969>3.0.CO;2-7 [doi]
PST - ppublish
SO  - Electrophoresis. 2000 Aug;21(14):2969-72. doi: 
      10.1002/1522-2683(20000801)21:14<2969::AID-ELPS2969>3.0.CO;2-7.