PMID- 11001921
OWN - NLM
STAT- MEDLINE
DCOM- 20001025
LR  - 20231213
IS  - 0006-4971 (Print)
IS  - 0006-4971 (Linking)
VI  - 96
IP  - 7
DP  - 2000 Oct 1
TI  - Maturation of dendritic cells leads to up-regulation of cellular FLICE-inhibitory 
      protein and concomitant down-regulation of death ligand-mediated apoptosis.
PG  - 2628-31
AB  - Dendritic cells (DCs) disappear from lymph nodes 1 to 2 days after antigen 
      presentation, presumably by apoptosis. To evaluate the role of death ligands in 
      elimination of DCs, we analyzed the sensitivity of human DCs to CD95 ligand 
      (CD95L) and tumor necrosis factor-related apoptosis-inducing ligand (TRAIL). We 
      found mature DCs to be resistant to killing via CD95L or TRAIL, whereas only 
      immature DCs were partially sensitive. However, all DC populations expressed 
      CD95, TRAIL-R2, and TRAIL-R3 at comparable levels, suggesting that sensitivity to 
      death ligand-induced DC apoptosis is not regulated at the receptor level. 
      Interestingly, mature DCs highly expressed the caspase 8 inhibitory protein 
      cFLIP, whereas only low levels were detected in immature DCs. Thus, death ligand 
      sensitivity proved to be dependent on DC maturation and inversely correlated with 
      expression levels of cFLIP. Induction of apoptosis by TRAIL or CD95L does not 
      seem to play a role in the elimination of mature DCs, but instead might serve to 
      regulate immature DC populations.
FAU - Leverkus, M
AU  - Leverkus M
AD  - University of Wurzburg Medical School, Department of Dermatology, Wurzburg, 
      Germany.
FAU - Walczak, H
AU  - Walczak H
FAU - McLellan, A
AU  - McLellan A
FAU - Fries, H W
AU  - Fries HW
FAU - Terbeck, G
AU  - Terbeck G
FAU - Brocker, E B
AU  - Brocker EB
FAU - Kampgen, E
AU  - Kampgen E
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Blood
JT  - Blood
JID - 7603509
RN  - 0 (Antigens, CD)
RN  - 0 (Apoptosis Regulatory Proteins)
RN  - 0 (Immunoglobulins)
RN  - 0 (Interleukin-1)
RN  - 0 (Interleukin-6)
RN  - 0 (Membrane Glycoproteins)
RN  - 0 (TNF-Related Apoptosis-Inducing Ligand)
RN  - 0 (TNFSF10 protein, human)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 0 (fas Receptor)
RN  - 207137-56-2 (Interleukin-4)
RN  - 83869-56-1 (Granulocyte-Macrophage Colony-Stimulating Factor)
RN  - EC 3.4.22.- (CASP8 protein, human)
RN  - EC 3.4.22.- (CASP9 protein, human)
RN  - EC 3.4.22.- (Caspase 8)
RN  - EC 3.4.22.- (Caspase 9)
RN  - EC 3.4.22.- (Caspases)
RN  - K7Q1JQR04M (Dinoprostone)
SB  - IM
MH  - Antigens, CD
MH  - *Apoptosis
MH  - Apoptosis Regulatory Proteins
MH  - Caspase 8
MH  - Caspase 9
MH  - Caspases/metabolism
MH  - Cells, Cultured
MH  - Dendritic Cells/chemistry/*physiology
MH  - Dinoprostone/pharmacology
MH  - Drug Resistance
MH  - Granulocyte-Macrophage Colony-Stimulating Factor/pharmacology
MH  - Humans
MH  - Immunoglobulins/analysis
MH  - Interleukin-1/pharmacology
MH  - Interleukin-4/pharmacology
MH  - Interleukin-6/pharmacology
MH  - Membrane Glycoproteins/analysis/*pharmacology
MH  - TNF-Related Apoptosis-Inducing Ligand
MH  - Tumor Necrosis Factor-alpha/analysis/*pharmacology
MH  - fas Receptor/analysis/*pharmacology
MH  - CD83 Antigen
EDAT- 2000/09/26 11:00
MHDA- 2001/02/28 10:01
CRDT- 2000/09/26 11:00
PHST- 2000/09/26 11:00 [pubmed]
PHST- 2001/02/28 10:01 [medline]
PHST- 2000/09/26 11:00 [entrez]
AID - S0006-4971(20)54378-4 [pii]
PST - ppublish
SO  - Blood. 2000 Oct 1;96(7):2628-31.