PMID- 11002288
OWN - NLM
STAT- MEDLINE
DCOM- 20001130
LR  - 20061115
IS  - 0360-4012 (Print)
IS  - 0360-4012 (Linking)
VI  - 62
IP  - 1
DP  - 2000 Oct 1
TI  - Neurofilament mRNAs are present and translated in the normal and severed sciatic 
      nerve.
PG  - 65-74
AB  - Local protein synthesis within axons has been studied on a limited scale. In the 
      present study, several techniques were used to investigate this synthesis in 
      sciatic nerve, and to show that it increases after damage to the axon. 
      Neurofilament (NF) mRNAs were probed by RT-PCR, Northern blot and in situ 
      hybridization in axons of intact rat sciatic nerve, and in proximal or distal 
      stumps after sciatic nerve transection. RT-PCR demonstrated the presence of NF-L, 
      NF-M and NF-H mRNAs in intact sciatic nerve, as well as in proximal and distal 
      stumps of severed nerves. Northern blot analysis of severed nerve detected NF-L 
      and NF-M, but not NF-H. This technique did not detect the three NFs mRNAs in 
      intact nerve. Detection of NF-L and NF-M mRNA in injured nerve, however, 
      indicated that there was an up-regulation in response to nerve injury. In situ 
      hybridization showed that NF-L mRNA was localized in the Schwann cell perinuclear 
      area, in the myelin sheath, and at the boundary between myelin sheath and 
      cortical axoplasm. RNA and protein synthesizing activities were always greater in 
      proximal as compared to distal stumps. NF triplet proteins were also shown to be 
      synthesized de novo in the proximal stump. The detection of neurofilament mRNAs 
      in nerves, their possible upregulation during injury and the synthesis of 
      neurofilament protein triplet in the proximal stumps, suggest that these mRNAs 
      may be involved in nerve regeneration, providing a novel point of view of this 
      phenomenon.
CI  - Copyright 2000 Wiley-Liss, Inc.
FAU - Sotelo-Silveira, J R
AU  - Sotelo-Silveira JR
AD  - Departamento de Biofisica, Instituto de Investigaciones Biologicas Clemente 
      Estable, Montevideo, Uruguay. sotelo@iibce.edu.ny
FAU - Calliari, A
AU  - Calliari A
FAU - Kun, A
AU  - Kun A
FAU - Benech, J C
AU  - Benech JC
FAU - Sanguinetti, C
AU  - Sanguinetti C
FAU - Chalar, C
AU  - Chalar C
FAU - Sotelo, J R
AU  - Sotelo JR
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Neurosci Res
JT  - Journal of neuroscience research
JID - 7600111
RN  - 0 (Neurofilament Proteins)
RN  - 0 (RNA, Messenger)
RN  - 0 (Ribosomal Proteins)
RN  - 0 (neurofilament protein L)
RN  - 0 (ribosomal protein S1)
SB  - IM
MH  - Animals
MH  - Autoradiography
MH  - Axotomy
MH  - Blotting, Northern
MH  - In Situ Hybridization
MH  - Male
MH  - Neurofilament Proteins/*genetics/*metabolism
MH  - Protein Biosynthesis/*genetics
MH  - RNA, Messenger/*metabolism
MH  - Rats
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Ribosomal Proteins/metabolism
MH  - Schwann Cells/cytology/metabolism
MH  - Sciatic Nerve/*metabolism/surgery
MH  - Up-Regulation
EDAT- 2000/09/26 11:00
MHDA- 2001/02/28 10:01
CRDT- 2000/09/26 11:00
PHST- 2000/09/26 11:00 [pubmed]
PHST- 2001/02/28 10:01 [medline]
PHST- 2000/09/26 11:00 [entrez]
AID - 10.1002/1097-4547(20001001)62:1<65::AID-JNR7>3.0.CO;2-Z [pii]
AID - 10.1002/1097-4547(20001001)62:1<65::AID-JNR7>3.0.CO;2-Z [doi]
PST - ppublish
SO  - J Neurosci Res. 2000 Oct 1;62(1):65-74. doi: 
      10.1002/1097-4547(20001001)62:1<65::AID-JNR7>3.0.CO;2-Z.