PMID- 11004405
OWN - NLM
STAT- MEDLINE
DCOM- 20001201
LR  - 20181130
IS  - 0378-1097 (Print)
IS  - 0378-1097 (Linking)
VI  - 191
IP  - 1
DP  - 2000 Oct 1
TI  - A novel technique for monitoring the development of bacterial biofilms in human 
      periodontal pockets.
PG  - 95-101
AB  - A new technique is presented for analyzing subgingival bacterial plaque. 
      Different materials (polytetrafluoroethylene, gold, dentin) kept for several days 
      in periodontal pockets of patients suffering from periodontitis were analyzed by 
      electron microscopy and fluorescence in situ hybridization (FISH). Those parts of 
      the carriers extending into the deepest zone of the pockets were predominantly 
      colonized by spirochetes and Gram-negative bacteria whereas those segments in 
      contact with a shallower region were colonized by streptococci. Independent of 
      the material used, the bacterial colonization of the carriers appears to be 
      similar. FISH using eubacteria- and species-specific oligonucleotides on 
      semi-thin cross-sections of the carriers in combination with confocal laser 
      scanning microscopy allowed detailed analysis of the architecture of biofilms and 
      identification of putative periodontal pathogens with single cell resolution.
FAU - Wecke, J
AU  - Wecke J
AD  - Robert Koch-Institut, Nordufer 20, Berlin, Germany. hanc@rki.de
FAU - Kersten, T
AU  - Kersten T
FAU - Madela, K
AU  - Madela K
FAU - Moter, A
AU  - Moter A
FAU - Gobel, U B
AU  - Gobel UB
FAU - Friedmann, A
AU  - Friedmann A
FAU - Bernimoulin, J
AU  - Bernimoulin J
LA  - eng
PT  - Journal Article
PL  - England
TA  - FEMS Microbiol Lett
JT  - FEMS microbiology letters
JID - 7705721
RN  - 7440-57-5 (Gold)
RN  - 9002-84-0 (Polytetrafluoroethylene)
SB  - IM
MH  - Bacteria/*growth & development/isolation & purification
MH  - Bacteriological Techniques
MH  - Biofilms/*growth & development
MH  - Dental Plaque/microbiology
MH  - Dentin
MH  - Gingiva/microbiology
MH  - Gold
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Microscopy, Fluorescence
MH  - Periodontal Pocket/*microbiology
MH  - Polytetrafluoroethylene
EDAT- 2000/09/27 11:00
MHDA- 2001/02/28 10:01
CRDT- 2000/09/27 11:00
PHST- 2000/09/27 11:00 [pubmed]
PHST- 2001/02/28 10:01 [medline]
PHST- 2000/09/27 11:00 [entrez]
AID - S0378109700003761 [pii]
AID - 10.1111/j.1574-6968.2000.tb09324.x [doi]
PST - ppublish
SO  - FEMS Microbiol Lett. 2000 Oct 1;191(1):95-101. doi: 
      10.1111/j.1574-6968.2000.tb09324.x.