PMID- 11004504
OWN - NLM
STAT- MEDLINE
DCOM- 20001019
LR  - 20190610
IS  - 0006-3002 (Print)
IS  - 0006-3002 (Linking)
VI  - 1492
IP  - 2-3
DP  - 2000 Jul 24
TI  - The genomic structure and promoter analysis of the human ABF-1 gene.
PG  - 320-9
AB  - The human ABF-1 gene is expressed in activated B-cells and Epstein-Barr 
      virus-immortalized lymphoblastoid cell lines. ABF-1 represents the only member 
      belonging to the basic helix-loop-helix (bHLH) family of transcription factors 
      whose expression pattern is restricted to B-cells. ABF-1 forms heterodimeric 
      complexes with E2A to modulate gene transcription. We report the cloning and 
      characterization of the human ABF-1 gene and the promoter region. The gene spans 
      more than 3 kb and contains two exons. Exon 1 contains 274 bp of a 
      5'-untranslated sequence (UTR) while exon 2 contains 1097 bp of 3'-UTR. Promoter 
      analysis of the 5'-flanking region revealed no apparent B-cell-restricted control 
      elements within approximately 700 bp, but clearly demonstrated the presence of a 
      functional minimal promoter residing immediately upstream of the transcription 
      start site. Analysis of the region containing the minimal promoter activity 
      identified no CCAAT or TATA sequence. Lastly, we have assigned the ABF-1 gene to 
      human chromosome 8q21.1 using fluorescence in situ hybridization (FISH). The 
      cloning of the human ABF-1 gene will facilitate further biochemical and genetic 
      studies of its function in the regulation of B-cell differentiation.
FAU - Mitchell, B
AU  - Mitchell B
AD  - Department of Biology, University of the Pacific, Stockton, CA 95211, USA.
FAU - Mugiya, M
AU  - Mugiya M
FAU - Youngblom, J
AU  - Youngblom J
FAU - Funes-Duran, M
AU  - Funes-Duran M
FAU - Miller, R
AU  - Miller R
FAU - Ezpeleta, J
AU  - Ezpeleta J
FAU - Rigby, N
AU  - Rigby N
FAU - Vierra, C
AU  - Vierra C
LA  - eng
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - Netherlands
TA  - Biochim Biophys Acta
JT  - Biochimica et biophysica acta
JID - 0217513
RN  - 0 (ABF1 protein, S cerevisiae)
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (Saccharomyces cerevisiae Proteins)
RN  - 0 (Transcription Factors)
RN  - 9007-49-2 (DNA)
SB  - IM
MH  - Base Sequence
MH  - Chromosome Mapping
MH  - Chromosomes, Human, Pair 8
MH  - DNA/analysis
MH  - DNA-Binding Proteins/*genetics
MH  - Genome, Human
MH  - Genomic Library
MH  - Humans
MH  - Karyotyping
MH  - Molecular Sequence Data
MH  - Promoter Regions, Genetic/*genetics
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - *Saccharomyces cerevisiae Proteins
MH  - TATA Box
MH  - Transcription Factors/*genetics
MH  - *Transcriptional Activation
MH  - Tumor Cells, Cultured
EDAT- 2000/09/27 11:00
MHDA- 2000/10/21 11:01
CRDT- 2000/09/27 11:00
PHST- 2000/09/27 11:00 [pubmed]
PHST- 2000/10/21 11:01 [medline]
PHST- 2000/09/27 11:00 [entrez]
AID - S0167-4781(00)00109-3 [pii]
AID - 10.1016/s0167-4781(00)00109-3 [doi]
PST - ppublish
SO  - Biochim Biophys Acta. 2000 Jul 24;1492(2-3):320-9. doi: 
      10.1016/s0167-4781(00)00109-3.