PMID- 11005247
OWN - NLM
STAT- MEDLINE
DCOM- 20010202
LR  - 20180604
IS  - 0213-3911 (Print)
IS  - 0213-3911 (Linking)
VI  - 15
IP  - 4
DP  - 2000 Oct
TI  - Use of interphase cytogenetics in demonstrating specific chromosomal aberrations 
      in solid tumors--new insights in the pathogenesis of malignant melanoma and head 
      and neck squamous cell carcinoma.
PG  - 1225-31
LID - 10.14670/HH-15.1225 [doi]
AB  - The detection of structural and numerical chromosomal aberrations is an important 
      part of the characterization of tumors and genetic diseases. The direct 
      demonstration of DNA sequences in interphase nuclei and metaphases by 
      fluorescence in situ hybridization (FISH) has been termed interphase 
      cytogenetics. It has been proven as a powerful technique to detect specific 
      aberrations in a wide variety of cell types, including paraffin-embedded tissue. 
      Nowadays a standard method in leukemia and lymphoma, interphase cytogenetics 
      contributes mainly to the diagnosis in these tumors and helps to classify soft 
      tissue tumors. Therefore FISH is mandatory for the choice of therapy in these 
      tumors. In contrast to the aforementioned, up to now, the value of FISH in solid 
      tumors is mostly limited to pure research and contributes in this way to our 
      understanding of tumor biology. But with the use of paraffin-embedded tissue and 
      the first results obtained, it seems very likely that a direct correlation 
      between histological classification and cytogenetic characteristics of solid 
      tumors can be achieved in the near future. This information might not only 
      provide insights into tumor biology, but could also contribute to a different 
      tumor classification, a sort of risk estimation, where we might predict the 
      possible biological behavior of solid tumors. This could greatly influence 
      further therapeutic decisions thus establishing the FISH technique as an 
      indisputable part in the diagnosis of solid tumors.
FAU - Poetsch, M
AU  - Poetsch M
AD  - Institute of Forensic Medicine, University of Greifswald, Germany. 
      poetsch@mail.uni-greifswald.de
FAU - Dittberner, T
AU  - Dittberner T
FAU - Woenckhaus, C
AU  - Woenckhaus C
FAU - Kleist, B
AU  - Kleist B
LA  - eng
PT  - Journal Article
PT  - Review
PL  - Spain
TA  - Histol Histopathol
JT  - Histology and histopathology
JID - 8609357
SB  - IM
MH  - Animals
MH  - Chromosome Aberrations/*genetics
MH  - Cytogenetics/*methods
MH  - Head and Neck Neoplasms/*pathology
MH  - Humans
MH  - Interphase/*genetics
MH  - Melanoma/*pathology
MH  - Neoplasms/*pathology
MH  - Neoplasms, Squamous Cell/*pathology
RF  - 71
EDAT- 2000/09/27 11:00
MHDA- 2001/03/03 10:01
CRDT- 2000/09/27 11:00
PHST- 2000/09/27 11:00 [pubmed]
PHST- 2001/03/03 10:01 [medline]
PHST- 2000/09/27 11:00 [entrez]
AID - 10.14670/HH-15.1225 [doi]
PST - ppublish
SO  - Histol Histopathol. 2000 Oct;15(4):1225-31. doi: 10.14670/HH-15.1225.