PMID- 11005571
OWN - NLM
STAT- MEDLINE
DCOM- 20010126
LR  - 20190607
IS  - 1522-8002 (Print)
IS  - 1476-5586 (Electronic)
IS  - 1476-5586 (Linking)
VI  - 2
IP  - 4
DP  - 2000 Jul-Aug
TI  - HGF/SF activates glycolysis and oxidative phosphorylation in DA3 murine mammary 
      cancer cells.
PG  - 365-77
AB  - Hepatocyte growth factor/scatter factor (HGF/SF) is a paracrine growth factor 
      which increases cellular motility and has also been implicated in tumor 
      development and progression and in angiogenesis. Little is known about the 
      metabolic alteration induced in cells following Met-HGF/SF signal transduction. 
      The hypothesis that HGF/SF alters the energy metabolism of cancer cells was 
      investigated in perfused DA3 murine mammary cancer cells by nuclear magnetic 
      resonance (NMR) spectroscopy, oxygen and glucose consumption assays and confocal 
      laser scanning microscopy (CLSM). 31P NMR demonstrated that HGF/SF induced 
      remarkable alterations in phospholipid metabolites, and enhanced the rate of 
      glucose phosphorylation (P < .05). 13C NMR measurements, using 
      [13C1]-glucose-enriched medium, showed that HGS/SF reduced the steady state 
      levels of glucose and elevated those of lactate (P < .05). In addition, HGF/SF 
      treatment increased oxygen consumption from 0.58+/-0.02 to 0.71+/-0.03 
      micromol/hour per milligram protein (P < .05). However, it decreased CO2 levels, 
      and attenuated pH decrease. The mechanisms of these unexpected effects were 
      delineated by CLSM, using NAD(P)H fluorescence measurements, which showed that 
      HGF/SF increased the oxidation of the mitochondrial NAD system. We propose that 
      concomitant with induction of ruffling, HGF/SF enhances both the glycolytic and 
      oxidative phosphorylation pathways of energy production.
FAU - Kaplan, O
AU  - Kaplan O
AD  - School of Chemistry, Tel-Aviv University, Ramat Aviv, Israel.
FAU - Firon, M
AU  - Firon M
FAU - Vivi, A
AU  - Vivi A
FAU - Navon, G
AU  - Navon G
FAU - Tsarfaty, I
AU  - Tsarfaty I
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Neoplasia
JT  - Neoplasia (New York, N.Y.)
JID - 100886622
RN  - 0 (Glucosephosphates)
RN  - 0 (Phospholipids)
RN  - 0U46U6E8UK (NAD)
RN  - 53-59-8 (NADP)
RN  - 67256-21-7 (Hepatocyte Growth Factor)
SB  - IM
MH  - Adenocarcinoma
MH  - Animals
MH  - Female
MH  - Glucosephosphates/metabolism
MH  - Glycolysis/*drug effects
MH  - Hepatocyte Growth Factor/*pharmacology
MH  - Mammary Neoplasms, Experimental
MH  - Mice
MH  - Models, Biological
MH  - NAD/metabolism
MH  - NADP/metabolism
MH  - Oxidative Phosphorylation/*drug effects
MH  - Phospholipids/metabolism
MH  - Signal Transduction
MH  - Tumor Cells, Cultured
PMC - PMC1550289
EDAT- 2000/09/27 11:00
MHDA- 2001/02/28 10:01
PMCR- 2000/07/01
CRDT- 2000/09/27 11:00
PHST- 2000/09/27 11:00 [pubmed]
PHST- 2001/02/28 10:01 [medline]
PHST- 2000/09/27 11:00 [entrez]
PHST- 2000/07/01 00:00 [pmc-release]
AID - 0103a [pii]
AID - 10.1038/sj.neo.7900103 [doi]
PST - ppublish
SO  - Neoplasia. 2000 Jul-Aug;2(4):365-77. doi: 10.1038/sj.neo.7900103.