PMID- 11007493
OWN - NLM
STAT- MEDLINE
DCOM- 20010202
LR  - 20190831
IS  - 0009-5915 (Print)
IS  - 0009-5915 (Linking)
VI  - 109
IP  - 5
DP  - 2000
TI  - Structural evolution of the germ line-limited chromosomes in Acricotopus.
PG  - 343-9
AB  - The elimination of chromatin or whole chromosomes from the future somatic nuclei 
      during germ line-soma differentiation in early embryogenesis is a genetic 
      phenomenon found in a wide variety of animal species. Less is known about the 
      origin, structure, and function of the germ line-limited chromosomes. In the 
      chironomid Acricotopus lucidus fluorescence in situ hybridization (FISH) with 
      labeled soma DNA to "Keimbahn" chromosomes (Ks) and soma chromosomes (Ss) of 
      spermatogonial mitoses revealed that each of the nine different K types possesses 
      large S-homologous sections, mostly in the distal parts of both chromosome arms. 
      Painting probes of the three Ss and of each of their chromosome arms were 
      generated by microdissection of polytene salivary gland chromosomes and 
      subsequent amplification by the degenerate oligonucleotide-primed polymerase 
      chain reaction. Multicolor FISH demonstrated that each of the Ks, with the 
      exception of one K type, was painted by only one of the three S probes. 
      Furthermore, in seven Ks, one chromosome arm was painted by the long-arm probe 
      and the other by the short-arm probe of the S concerned. The hybridization 
      pattern strongly suggests that each of these K types is derived from a specific 
      S. One function of the S-homologous K sections is thought to be determination of 
      the regular occurrence of crossover events, with the resulting chiasmata in these 
      sections ensuring correct segregation of the K homologs during meiosis. Reverse 
      chromosome painting on polytene S sets with a probe generated from metaphase Ks 
      corroborates the above results and produces conclusive evidence for the 
      hypothesis that during evolution the Ks have developed from the Ss by 
      endopolyploidization and rearrangements followed by the accumulation of germ 
      line-specific repetitive DNA sequences in the centromeric regions.
FAU - Staiber, W
AU  - Staiber W
AD  - Institute of Genetics, University of Hohenheim, Stuttgart, Germany. 
      wstaiber@uni-hohnheim.de
FAU - Schiffkowski, C
AU  - Schiffkowski C
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Austria
TA  - Chromosoma
JT  - Chromosoma
JID - 2985138R
RN  - 0 (DNA, Ribosomal)
SB  - IM
MH  - Animals
MH  - Cell Differentiation
MH  - Chironomidae/cytology/*genetics
MH  - Chromosome Banding
MH  - Chromosome Painting
MH  - Chromosomes/*ultrastructure
MH  - DNA, Ribosomal
MH  - Germ Cells/*cytology
EDAT- 2000/09/28 11:00
MHDA- 2001/03/03 10:01
CRDT- 2000/09/28 11:00
PHST- 2000/09/28 11:00 [pubmed]
PHST- 2001/03/03 10:01 [medline]
PHST- 2000/09/28 11:00 [entrez]
AID - 10.1007/s004120000084 [doi]
PST - ppublish
SO  - Chromosoma. 2000;109(5):343-9. doi: 10.1007/s004120000084.