PMID- 11007504 OWN - NLM STAT- MEDLINE DCOM- 20010202 LR - 20061115 IS - 0256-7040 (Print) IS - 0256-7040 (Linking) VI - 16 IP - 8 DP - 2000 Aug TI - Aberrant expression of neurotrophic factors in the ventricular progenitor cells of infant congenitally hydrocephalic rats. PG - 516-21 AB - OBJECTS: This study was conducted to investigate the roles of neurotrophic factors in the development of hydrocephalus in HTX rats. METHODS: Expressions of brain-derived neurotrophic factor (BDNF), neurotrophin-3 (NT-3), and fibroblast growth factor (FGF)-1 were examined immunohistochemically in the cerebral cortex and ventricular zone of 6-day-old rats with congenital hydrocephalus (HTX rats). In the ventricular zone of hydrocephalic rats, potent BDNF-like immunoreactivity (-LI) and weak but significant signals for NT-3- and FGF-1-LIs were observed. However, no significant signals were detected in non-HTX rats. A small subpopulation of ventricular cells was positive for microtubule-associated protein 2 in HTX and non-HTX rats. The positive cells in the HTX rats had neurites much longer than those in the non-HTX animals, suggesting that some ventricular cells of the hydrocephalics had ectopically differentiated into mature neurons. CONCLUSIONS: This abnormal differentiation may have been responsible for the aberrant expressions of neurotrophic factors. In contrast, the cerebral neuronal layers did not show such prominent alterations in neurotrophic factor expression. FAU - Fukumitsu, H AU - Fukumitsu H AD - Laboratory of Molecular Biology, Gifu Pharmaceutical University, Mitahota-higashi, Japan. FAU - Ohmiya, M AU - Ohmiya M FAU - Nitta, A AU - Nitta A FAU - Furukawa, S AU - Furukawa S FAU - Mima, T AU - Mima T FAU - Mori, K AU - Mori K LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't PL - Germany TA - Childs Nerv Syst JT - Child's nervous system : ChNS : official journal of the International Society for Pediatric Neurosurgery JID - 8503227 RN - 0 (Brain-Derived Neurotrophic Factor) RN - 0 (Neurotrophin 3) RN - 62031-54-3 (Fibroblast Growth Factors) SB - IM MH - Animals MH - Animals, Newborn MH - Brain-Derived Neurotrophic Factor/*metabolism MH - Cell Differentiation MH - Cerebral Cortex/metabolism MH - Cerebral Ventricles/*metabolism MH - Fibroblast Growth Factors/metabolism MH - Hydrocephalus/*metabolism MH - Immunohistochemistry MH - Models, Animal MH - Neurons/cytology MH - Neurotrophin 3/metabolism MH - Rats MH - Stem Cells/*metabolism EDAT- 2000/09/28 11:00 MHDA- 2001/03/03 10:01 CRDT- 2000/09/28 11:00 PHST- 2000/09/28 11:00 [pubmed] PHST- 2001/03/03 10:01 [medline] PHST- 2000/09/28 11:00 [entrez] AID - 10.1007/s003810000305 [doi] PST - ppublish SO - Childs Nerv Syst. 2000 Aug;16(8):516-21. doi: 10.1007/s003810000305.