PMID- 11010885
OWN - NLM
STAT- MEDLINE
DCOM- 20001130
LR  - 20210526
IS  - 0099-2240 (Print)
IS  - 1098-5336 (Electronic)
IS  - 0099-2240 (Linking)
VI  - 66
IP  - 10
DP  - 2000 Oct
TI  - Bacterial origin and community composition in the barley phytosphere as a 
      function of habitat and presowing conditions.
PG  - 4372-7
AB  - An understanding of the factors influencing colonization of the rhizosphere is 
      essential for improved establishment of biocontrol agents. The aim of this study 
      was to determine the origin and composition of bacterial communities in the 
      developing barley (Hordeum vulgare) phytosphere, using denaturing gradient gel 
      electrophoresis (DGGE) analysis of 16S rRNA genes amplified from extracted DNA. 
      Discrete community compositions were identified in the endorhizosphere, 
      rhizoplane, and rhizosphere soil of plants grown in an agricultural soil for up 
      to 36 days. Cluster analysis revealed that DGGE profiles of the rhizoplane more 
      closely resembled those in the soil than the profiles found in the root tissue or 
      on the seed, suggesting that rhizoplane bacteria primarily originated from the 
      surrounding soil. No change in bacterial community composition was observed in 
      relation to plant age. Pregermination of the seeds for up to 6 days improved the 
      survival of seed-associated bacteria on roots grown in soil, but only in the 
      upper, nongrowing part of the rhizoplane. The potential occurrence of skewed PCR 
      amplification was examined, and only minor cases of PCR bias for mixtures of two 
      different DNA samples were observed, even when one of the samples contained plant 
      DNA. The results demonstrate the application of culture-independent, molecular 
      techniques in assessment of rhizosphere bacterial populations and the importance 
      of the indigenous soil population in colonization of the rhizosphere.
FAU - Normander, B
AU  - Normander B
AD  - Department of Microbial Ecology and Biotechnology, National Environmental 
      Research Institute, DK-4000 Roskilde, Denmark. bn@dmu.dk
FAU - Prosser, J I
AU  - Prosser JI
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Appl Environ Microbiol
JT  - Applied and environmental microbiology
JID - 7605801
RN  - 0 (DNA, Bacterial)
RN  - 0 (DNA, Ribosomal)
RN  - 0 (RNA, Bacterial)
RN  - 0 (RNA, Ribosomal, 16S)
SB  - IM
MH  - Bacteria/*classification/genetics/isolation & purification
MH  - Cluster Analysis
MH  - DNA, Bacterial/genetics/isolation & purification
MH  - DNA, Ribosomal/genetics/isolation & purification
MH  - Electrophoresis, Polyacrylamide Gel/methods
MH  - Hordeum/*microbiology
MH  - Molecular Sequence Data
MH  - Polymerase Chain Reaction/methods
MH  - RNA, Bacterial/genetics
MH  - RNA, Ribosomal, 16S/genetics
MH  - Soil Microbiology
PMC - PMC92311
EDAT- 2000/09/30 11:00
MHDA- 2001/02/28 10:01
PMCR- 2000/10/01
CRDT- 2000/09/30 11:00
PHST- 2000/09/30 11:00 [pubmed]
PHST- 2001/02/28 10:01 [medline]
PHST- 2000/09/30 11:00 [entrez]
PHST- 2000/10/01 00:00 [pmc-release]
AID - 0284 [pii]
AID - 10.1128/AEM.66.10.4372-4377.2000 [doi]
PST - ppublish
SO  - Appl Environ Microbiol. 2000 Oct;66(10):4372-7. doi: 
      10.1128/AEM.66.10.4372-4377.2000.