PMID- 11010908 OWN - NLM STAT- MEDLINE DCOM- 20001130 LR - 20210526 IS - 0099-2240 (Print) IS - 1098-5336 (Electronic) IS - 0099-2240 (Linking) VI - 66 IP - 10 DP - 2000 Oct TI - Use of combined microautoradiography and fluorescence in situ hybridization to determine carbon metabolism in mixed natural communities of uncultured bacteria from the genus Achromatium. PG - 4518-22 AB - Combined microautoradiography and fluorescence in situ hybridization (FISH) was used to investigate carbon metabolism in uncultured bacteria from the genus Achromatium. All of the Achromatium species identified in a freshwater sediment from Rydal Water, Cumbria, United Kingdom, which were distinguishable only by FISH, assimilated both [(14)C]bicarbonate and [(14)C]acetate. This extends previous findings that Achromatium spp. present at another location could only utilize organic carbon sources. Achromatium spp., therefore, probably exhibit a range of physiologies, i.e., facultative chemolithoautotrophy, mixotrophy, and chemoorganoheterotrophy, similar to other large sulfur bacteria (e.g., Beggiatoa spp.). FAU - Gray, N D AU - Gray ND AD - Fossil Fuels and Environmental Geochemistry Postgraduate Institute and Centre for Molecular Ecology, University of Newcastle, Newcastle upon Tyne NE1 7RU, United Kingdom. FAU - Howarth, R AU - Howarth R FAU - Pickup, R W AU - Pickup RW FAU - Jones, J G AU - Jones JG FAU - Head, I M AU - Head IM LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't PL - United States TA - Appl Environ Microbiol JT - Applied and environmental microbiology JID - 7605801 RN - 0 (Acetates) RN - 0 (Bicarbonates) RN - 0 (Carbon Radioisotopes) RN - 7440-44-0 (Carbon) SB - IM MH - Acetates/*metabolism MH - Autoradiography/methods MH - Bicarbonates/*metabolism MH - Carbon/*metabolism MH - Carbon Radioisotopes MH - Fresh Water/*microbiology MH - Geologic Sediments/*microbiology MH - Gram-Negative Aerobic Bacteria/*classification/isolation & purification/*metabolism MH - In Situ Hybridization, Fluorescence MH - *Phylogeny PMC - PMC92334 EDAT- 2000/09/30 11:00 MHDA- 2001/02/28 10:01 PMCR- 2000/10/01 CRDT- 2000/09/30 11:00 PHST- 2000/09/30 11:00 [pubmed] PHST- 2001/02/28 10:01 [medline] PHST- 2000/09/30 11:00 [entrez] PHST- 2000/10/01 00:00 [pmc-release] AID - 0476 [pii] AID - 10.1128/AEM.66.10.4518-4522.2000 [doi] PST - ppublish SO - Appl Environ Microbiol. 2000 Oct;66(10):4518-22. doi: 10.1128/AEM.66.10.4518-4522.2000.