PMID- 11011696
OWN - NLM
STAT- MEDLINE
DCOM- 20010202
LR  - 20190831
IS  - 0301-4622 (Print)
IS  - 0301-4622 (Linking)
VI  - 86
IP  - 1
DP  - 2000 Jul 31
TI  - Effect of Adriamycin on the boundary lipid structure of cytochrome c oxidase: 
      pico-second time-resolved fluorescence depolarization studies.
PG  - 15-28
AB  - The fluorescence dynamics of the dye 3,3'-diethyloxadicarbocyanine iodide (DODCI) 
      was used to probe the microenvironment of cytochrome c oxidase (CcO) and 
      cardiolipin. The dye was partitioned between an aqueous and a hydrophobic phase. 
      The 'bound' and 'free' populations of DODCI could be separated by analysis of the 
      time-resolved fluorescence decay of the dye. The anisotropy decay of the DODCI 
      bound to CcO showed a unique 'dip and rise' shape that was analyzed by a 
      combination of rotational correlation times with time-dependent weight factors 
      for each lifetime component. Rotational dynamics studies revealed the existence 
      of a restricted motion of the dye bound at the enzyme surface. Adriamycin, an 
      anticancer, albeit cardiotoxic drug, was previously proposed to affect the 
      surface structure of CcO, most likely by causing a disorder to the surface lipid 
      arrangement. A drastic change in the rotational correlation time of the dye bound 
      to the enzyme surface was observed, which suggested a depletion of cardiolipin 
      layer due to complexation with the drug. The effect of Adriamycin on cardiolipin 
      was drastic, leading to its phase separation. The present study suggests that the 
      effect of Adriamycin on CcO is primarily a segregation of the cardiolipins.
FAU - Das, T K
AU  - Das TK
AD  - Albert Einstein College of Medicine, Bronx, NY 10461, USA. tdas@aecom.yu.edu
FAU - Mazumdar, S
AU  - Mazumdar S
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Biophys Chem
JT  - Biophysical chemistry
JID - 0403171
RN  - 0 (Antibiotics, Antineoplastic)
RN  - 0 (Cardiolipins)
RN  - 0 (Detergents)
RN  - 0 (Lipids)
RN  - 0 (Phosphatidylcholines)
RN  - 0 (Phospholipids)
RN  - 69279-91-0 (asolectin)
RN  - 80168379AG (Doxorubicin)
RN  - EC 1.9.3.1 (Electron Transport Complex IV)
SB  - IM
MH  - Algorithms
MH  - Animals
MH  - Anisotropy
MH  - Antibiotics, Antineoplastic/*chemistry
MH  - Cardiolipins/chemistry
MH  - Cattle
MH  - Chemical Phenomena
MH  - Chemistry, Physical
MH  - Detergents
MH  - Doxorubicin/*chemistry
MH  - Electron Transport Complex IV/*chemistry
MH  - Fluorescence Polarization Immunoassay
MH  - Lipids/*chemistry
MH  - Phosphatidylcholines
MH  - Phospholipids/chemistry
EDAT- 2000/09/30 11:00
MHDA- 2001/03/03 10:01
CRDT- 2000/09/30 11:00
PHST- 2000/09/30 11:00 [pubmed]
PHST- 2001/03/03 10:01 [medline]
PHST- 2000/09/30 11:00 [entrez]
AID - S0301462200001587 [pii]
AID - 10.1016/s0301-4622(00)00158-7 [doi]
PST - ppublish
SO  - Biophys Chem. 2000 Jul 31;86(1):15-28. doi: 10.1016/s0301-4622(00)00158-7.