PMID- 11011822 OWN - NLM STAT- MEDLINE DCOM- 20010125 LR - 20131121 IS - 0883-9441 (Print) IS - 0883-9441 (Linking) VI - 15 IP - 3 DP - 2000 Sep TI - Influence of amrinone on intestinal villus blood flow during endotoxemia. PG - 97-102 AB - PURPOSE: The objective of this study was to determine the effects of a continuous infusion of the phosphodiesterase (PDE) inhibitor amrinone on mucosal villus blood flow in a normotensive model of endotoxemia. MATERIALS AND METHODS: Twenty-four anesthetized and ventilated rats underwent laparotomy, and an ileal portion was exteriorized and opened by an antimesenteric incision. The ileal segment was fixed on a plexiglass stage with the mucosal surface upward. Microcirculatory parameters were assessed by intravital videomicroscopy. The animals were randomly assigned to receive one of three treatments: infusion of Escherichia coli lipopolysaccharides (LPS, 2 mg/kg/h) without phosphodiesterase inhibitor pretreatment (LPS group); or infusion of LPS with amrinone pretreatment (40 microg/kg/min, start 30 minutes before LPS infusion) (amrinone group), or infusion of equivalent volumes of NaCl 0.9% (control group). Macrohemodynamic parameters (MAP, HR) and microhemodynamic parameters of ileal mucosa (mean diameter of central arterioles = D(A) and mean erythrocyte velocity within the arterioles = VE) were measured 30 minutes before and at 0, 60, and 120 minutes after induction of endotoxemia. Mucosal villus blood flow was calculated from D(A) and VE. RESULTS: In this normotensive endotoxemia model, MAP remained stable in the control and the LPS group but significantly decreased in the amrinone group.The endotoxin-induced decrease of V(E) and D(A) of central arterioles of mucosal villi could be attenuated and prevented, respectively. Thus, the endotoxin-induced decrease of mucosal villus blood flow was diminished but not fully restored by amrinone infusion. CONCLUSION: Our results indicate that amrinone during an early stage of sepsis is of limited value. It attenuates mucosal hypoperfusion but contributes to systemic hypotension. FAU - Schmidt, W AU - Schmidt W AD - Department of Anesthesiology, University of Heidelberg, Germany. FAU - Tinelli, M AU - Tinelli M FAU - Secchi, A AU - Secchi A FAU - Gebhard, M M AU - Gebhard MM FAU - Martin, E AU - Martin E FAU - Schmidt, H AU - Schmidt H LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't PL - United States TA - J Crit Care JT - Journal of critical care JID - 8610642 RN - 0 (Phosphodiesterase Inhibitors) RN - JUT23379TN (Amrinone) SB - IM MH - Amrinone/*pharmacology/therapeutic use MH - Analysis of Variance MH - Animals MH - Endotoxemia/*drug therapy MH - Hemodynamics/drug effects MH - Intestinal Mucosa/*blood supply/*drug effects MH - Male MH - Microcirculation/drug effects MH - Phosphodiesterase Inhibitors/*pharmacology/therapeutic use MH - Random Allocation MH - Rats MH - Rats, Wistar EDAT- 2000/09/30 11:00 MHDA- 2001/02/28 10:01 CRDT- 2000/09/30 11:00 PHST- 2000/09/30 11:00 [pubmed] PHST- 2001/02/28 10:01 [medline] PHST- 2000/09/30 11:00 [entrez] AID - S0883-9441(00)31390-9 [pii] AID - 10.1053/jcrc.2000.16462 [doi] PST - ppublish SO - J Crit Care. 2000 Sep;15(3):97-102. doi: 10.1053/jcrc.2000.16462.