PMID- 11012220
OWN - NLM
STAT- MEDLINE
DCOM- 20001102
LR  - 20081121
IS  - 1066-5099 (Print)
IS  - 1066-5099 (Linking)
VI  - 14 Suppl 1
DP  - 1996
TI  - The platelet glycoprotein Ib-V-IX system: regulation of gene expression.
PG  - 188-93
AB  - Platelet glycoproteins (GPs) Ib-V-IX form the surface receptor for von Willebrand 
      factor, and this receptor-ligand interaction mediates the shear-dependent 
      adhesion of platelets to damaged arterial vessel walls. The receptor is a 
      multicomponent structure consisting of four distinct polypeptides (heterodimeric 
      GPIb: Ib alpha-Mr143k and Ib beta-Mr22k; GPV-Mr83k; GPIX-Mr20k), and each of the 
      four cDNAs and genes has been cloned and characterized. The genes appear to have 
      evolved from a common progenitor genomic sequence related to that encoding GPIX. 
      They share simple structures with few introns and possess common consensus 
      regulatory sequences (GATA, ets, Sp-1) in their 5' flanks. Both the GPIb alpha 
      and the GPIX promoters have been analyzed by transfection of reporter constructs 
      into hematopoietic and nonhematopoietic cells. The promoters function in a 
      tissue-specific fashion, and gel shift and mutational analyses indicate that GATA 
      and ets sequences regulate activity. In the case of the GPIX promoter, footprints 
      confirm the role of the ets-related consensus region. Recent studies of GPIb beta 
      transcriptional regulation suggest that an aberrant polyadenylation signal, 
      located in the 3' end of the gene immediately upstream of the GPIb beta gene, 
      allows in vitro expression of a rare extended fusion transcript encoding both the 
      upstream protein and GPIb beta. Little detailed information is available in 
      regard to expression of the GPV gene. In summary, the genes of the GPIb-V-IX 
      system display features of other megakaryocyte/platelet genes, but the unique 
      regulatory events that direct the selective expression of these genes in 
      megakaryocytes remain to be defined.
FAU - Roth, G J
AU  - Roth GJ
AD  - Hematology Section, Medical and Research Services, Seattle Veterans 
      Administration Medical Center and the University of Washington, 98108, USA.
FAU - Yagi, M
AU  - Yagi M
FAU - Bastian, L S
AU  - Bastian LS
LA  - eng
GR  - HL 09265/HL/NHLBI NIH HHS/United States
GR  - HL 39947/HL/NHLBI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PT  - Research Support, U.S. Gov't, P.H.S.
PT  - Review
PL  - England
TA  - Stem Cells
JT  - Stem cells (Dayton, Ohio)
JID - 9304532
RN  - 0 (DNA, Complementary)
RN  - 0 (Platelet Glycoprotein GPIb-IX Complex)
RN  - 0 (Recombinant Fusion Proteins)
RN  - 24937-83-5 (Poly A)
SB  - IM
MH  - Chromosome Mapping
MH  - DNA Mutational Analysis
MH  - DNA, Complementary/metabolism
MH  - Endothelium, Vascular/metabolism
MH  - *Gene Expression Regulation
MH  - Genes, Reporter
MH  - Hematopoietic Stem Cells/metabolism
MH  - Humans
MH  - Introns
MH  - Models, Biological
MH  - Platelet Aggregation/*genetics
MH  - Platelet Glycoprotein GPIb-IX Complex/chemistry/*genetics/*metabolism
MH  - Poly A/metabolism
MH  - Promoter Regions, Genetic
MH  - Recombinant Fusion Proteins/metabolism
MH  - Tissue Distribution
MH  - Transcription, Genetic
MH  - Transfection
RF  - 36
EDAT- 1996/01/01 00:00
MHDA- 2001/02/28 10:01
CRDT- 1996/01/01 00:00
PHST- 1996/01/01 00:00 [pubmed]
PHST- 2001/02/28 10:01 [medline]
PHST- 1996/01/01 00:00 [entrez]
AID - 10.1002/stem.5530140724 [doi]
PST - ppublish
SO  - Stem Cells. 1996;14 Suppl 1:188-93. doi: 10.1002/stem.5530140724.